Marei N.H.El-Mazny W.El-Shaer A.Zaki K.D.Hussein Z.S.Abd-El-Samie E.M.Nanotechnology ProgramSchool of Sciences and EngineeringAmerican University in CairoNew CairoEgypt; Research and Development UnitHolding Company for Biological products and Vaccines-VACSERAGizaEgypt; Biotechnology ProgramFaculty of SciencesCairo UniversityGizaEgypt; Faculty of BiotechnologyModern Sciences and Arts University6th of OctoberEgypt; Entomology DepartmentFaculty of SciencesCairo UniversityGizaEgypt2020-01-092020-01-0920171418130https://doi.org/10.1016/j.ijbiomac.2017.01.009PubMedID28062236https://t.ly/XAAVLScopusChitosan (CS) has received great attention in tissue engineering, especially in wound healing acceleration. In this study, chitin was isolated from desert locust (Schistocerca gregaria) and shrimp (Penaeus monodon) then deacetylated to chitosan. Then, chitosan was characterized by degree of deacetylation (DD), molecular weight (M.Wt), swelling index (SI), Fourier transform infrared (FTIR) and X ray diffraction (XRD). The chitosan was then casted into 2D scaffolds and was pictured using scanning electron microscope (SEM). In a comparative study, primary cell cultures of neonatal (1�2�day old) mice skin tissue, supplemented with 10% fetal calf serum, were seeded onto locust chitosan based scaffolds (LCSBS) and shrimp chitosan based scaffold (SCSBS). Their attachment percentage was determined after 1�h. The cell proliferation rate was tested for 5�days on LCSBS and SCSBS. Wound healing activity progress of LCSBS and SCSBS was tested in vivo using histopathology, and results revealed that seeded and unseeded LCSBS accelerated healing in contrast to SCSBS. The data demonstrated that LCSBS shows a high degree of biocompatibility in vivo. These results suggest that LCSBS is a potential substitute for the development of low cost implantable materials to accelerate wound healing. � 2017 Elsevier B.V.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityChitosanTissue engineeringWound healingchitinchitosanchitosananalytical parametersanimal cellanimal experimentanimal modelanimal tissueArticleBagg albino mousebiocompatibilitycell adhesioncell proliferationcomparative studycontrolled studydeacetylationelasticityfibroblasthistopathologyin vitro studyin vivo studyinfrared spectroscopylocust chitosan based scaffoldmalemolecular weightmousenewbornnonhumanPenaeus monodonphysical chemistryscanning electron microscopySchistocerca gregariashrimp chitosan based scaffoldswelling indextissue engineeringtissue scaffoldviscositywound healingX ray diffractionanimalCaeliferachemistrycytologydrug effectsPenaeidaeporosityskintissue scaffoldwound healingAnimalsCell AdhesionCell ProliferationChitosanGrasshoppersMicePenaeidaePorositySkinTissue ScaffoldsWound HealingArticlehttps://doi.org/10.1016/j.ijbiomac.2017.01.009PubMedID28062236