Salem H.Lotfy H.M.Hassan N.Y.El-Zeiny M.B.Saleh S.S.Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)11787 6th October CityEgypt; Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini Street11562 CairoEgypt; Analytical Chemistry DepartmentFaculty of PharmacyModern University for Technology and Information (MTI)12582 Al Hadaba Al WostaCairoEgypt2020-01-092020-01-09201513861425https://doi.org/10.1016/j.saa.2014.07.078PubMed ID 25171050https://t.ly/w18YEScopusMSA Google ScholarThis work represents a comparative study of different aspects of manipulating ratio spectra, which are: double divisor ratio spectra derivative (DR-DD), area under curve of derivative ratio (DR-AUC) and its novel approach, namely area under the curve correction method (AUCCM) applied for overlapped spectra; successive derivative of ratio spectra (SDR) and continuous wavelet transform (CWT) methods. The proposed methods represent different aspects of manipulating ratio spectra of the ternary mixture of Ofloxacin (OFX), Prednisolone acetate (PA) and Tetryzoline HCl (TZH) combined in eye drops in the presence of benzalkonium chloride as a preservative. The proposed methods were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulation containing the cited drugs. The proposed methods were validated according to the ICH guidelines. A comparative study was conducted between those methods regarding simplicity, limitation and sensitivity. The obtained results were statistically compared with those obtained from the reported HPLC method, showing no significant difference with respect to accuracy and precision.� 2014 Elsevier B.V. All rights reserved.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityArea under the curveDerivative of ratio spectraOfloxacinPrednisoloneTetryzolineWavelet transformChlorine compoundsMixturesArea under the curvesOfloxacinPrednisoloneRatio spectraTetryzolineWavelet transformseye dropsimidazole derivativeofloxacinprednisoloneprednisolone acetatetetryzolineanalogs and derivativesarea under the curvechemistrycomparative studyproceduresreproducibilityspectrophotometrystandardwavelet analysisArea Under CurveImidazolesOfloxacinOphthalmic SolutionsPrednisoloneReference StandardsReproducibility of ResultsSpectrophotometryWavelet AnalysisA comparative study of different aspects of manipulating ratio spectra applied for ternary mixtures: Derivative spectrophotometry versus wavelet transformArticlehttps://doi.org/10.1016/j.saa.2014.07.078PubMed ID 25171050