Lotfy H.M.Saleh S.S.El-Maraghy C.M.Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini StreetCairo11562Egypt; Pharmaceutical Chemistry DepartmentFaculty of Pharmaceutical Sciences & Pharmaceutical IndustriesFuture University in EgyptCairo12311Egypt; Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)11787 6th OctoberEgypt2020-01-092020-01-09202013861425https://doi.org/10.1016/j.saa.2019.117339PubMed ID 31344573https://t.ly/R0JbbScopusMSA Google ScholarAn analytical investigation was carried out to study the treatment and amplification of the spectral signals produced by critical concentrations with high accuracy and precision using two advanced approaches. The factorized-spectrum approach was applied through two novel methods which were: absorptivity centering technique via both: factorized zero order absorption spectrum (ACT-FSD0 ?A) and factorized ratio spectrum (ACT-FSR?P). The proposed methods were found to be linear in the ranges of (15�100 ?g/mL) and (3�40 ?g/mL) for ASP and MTO, respectively. Those methods were compared to the methods following the geometrical standard addition approach: ratio H-point standard addition method (RHPSAM) and geometrical induced amplitude modulation (GIAM). The approaches were applied for the determination of the minor component metoclopramide in its mixture with the major component aspirin in the challengeable ratio of (1,90) respectively in a white multicomponent system. The results obtained from the proposed approaches were statistically compared with each other. The methods were validated according to ICH guidelines where the results were found to be within the acceptable limits. The methods were found to be accurate and reliable for the determination of metoclopramide critical concentration besides aspirin concentration. The results of single factor ANOVA analysis indicated that there is no significant difference among the developed methods. These methods provided simple resolution of this binary combination from synthetic mixtures and pharmaceutical preparation and can be conveniently adopted for routine quality control analysis. � 2019 Elsevier B.V.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityAmplitude modulationAspirinFactorizedH-pointMetoclopramideAbsorption spectroscopyAdvanced AnalyticsAmplitude modulationBinary mixturesSpectrum analysisAnalytical investigationsAspirinFactorizedH-pointMetoclopramideMulti-component systemsPharmaceutical preparationsStandard addition methodQuality controlArticlehttps://doi.org/10.1016/j.saa.2019.117339PubMed ID 31344573