Yousry C.Fahmy R.H.Essam T.El-laithy H.M.Elkheshen S.A.Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyCairo UniversityKasr El-Aini StCairoEgypt; Department of Microbiology and Immunologyand Biotechnology CenterFaculty of PharmacyCairo UniversityKasr El-Aini StCairoEgypt; Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyOctober University for Modern Sciences and ArtsCairoEgypt; Department of Pharmaceutical TechnologyFaculty of Pharmaceutical Sciences and Pharmaceutical IndustriesFuture University in EgyptCairoEgypt2020-01-092020-01-0920163639045https://doi.org/10.3109/03639045.2016.1171335PubMed ID 27093938https://t.ly/mb8vrScopusContext: A microbiological multidistrict-based survey from different Egyptian governorates was conducted to determine the most prevalent causative agents of ocular infections in the Egyptian population. Antibiotic sensitivity testing was then performed to identify the most potent antimicrobial agent. Vancomycin (VCM) proved the highest activity against gram-positive Staphylococcus bacteria, which are the most commonly isolated causative agents of ocular infection. However, topically applied VCM suffers from poor ocular bioavailability because of its high molecular weight and hydrophilicity. Objective: The aim of the present study was to develop VCM-loaded solid lipid nanoparticles (SLNs) using water-in-oil-in-water (W/O/W) double emulsion, solvent evaporation technique to enhance ocular penetration and prolong ophthalmic residence of VCM. Method: Two consecutive full factorial designs (24 followed by 32) were adopted to study the effect of different formulation and process parameters on SLN formulation. The lipid type and structure, polyvinyl alcohol (PVA) molecular weight and concentration, sonication time, as well as lipid:drug ratio were studied as independent variables. The formulated SLN formulae were evaluated for encapsulation efficiency (EE%), particle size (PS), and zeta potential as dependent variables. Results: The statistically-optimized SLN formula (1:1 ratio of glyceryltripalmitate:VCM with 1% low molecular weight PVA and 1 min sonication time) had average PS of 277.25 nm, zeta potential of ?20.45, and 19.99% drug encapsulation. Scanning and transmission electron micrographs showed well-defined, spherical, homogenously distributed particles. Conclusion: The present study suggests that VCM incorporation into SLNs is successfully achievable; however, further studies with different nanoencapsulation materials and techniques would be valuable for improving VCM encapsulation. � 2016 Informa UK Limited, trading as Taylor & Francis Group.EnglishEgyptmulti-district microbiological surveyocular infectionsolid lipid nanoparticlesvancomycinamikacinamoxicillinamoxicillin plus clavulanic acidampicillinazithromycinaztreonamcefoperazonecefoperazone plus sulbactamcefotaximeciprofloxacinclarithromycincotrimoxazolegeluciregentamicinguanosine diphosphateguanosine phosphateimipenemlevofloxacinmeticillinmezlocillinnovobiocinoilpiperacillinpolyvinyl alcoholsolid lipid nanoparticlesultamicillintripalmitinvancomycinwateremulsionlipidnanoparticlevancomycinantibiotic sensitivityArticledrug formulationdrug penetrationevaporationintraocular drug administrationminimum inhibitory concentrationmolecular weightnanoencapsulationnanopharmaceuticsnonhumanparticle sizeStaphylococcuszeta potentialchemical phenomenachemistrydrug delivery systememulsioneyemicrobiological phenomena and functionsmicrobiologyproceduresultrasoundDrug Delivery SystemsEmulsionsEyeHydrophobic and Hydrophilic InteractionsLipidsMicrobiological PhenomenaNanoparticlesParticle SizePolyvinyl AlcoholSonicationVancomycinArticlehttps://doi.org/10.3109/03639045.2016.1171335PubMed ID 27093938