Mohamed A.A.Soliman H.Ismail M.Ziada D.Farid T.M.Aref A.M.Al Daly M.E.Abd Elmageed Z.Y.Departments of BiochemistryNational Hepatology and Tropical Medicine Research InstituteEgypt; Department of Tropical Medicine and Infectious DiseasesFaculty of MedicineTanta UniversityEgypt; Departments of SurgeryNational Hepatology and Tropical Medicine Research InstituteEgypt; Department of Clinical BiochemistryFaculty of MedicineKing Abdel Aziz UniversityJeddahSaudi Arabia; Department of Biological SciencesFaculty of DentistryModern Science and Arts UniversityEgypt; Clinical OncologyFaculty of MedicineCairo UniversityEgypt; Tulane University Health Sciences CenterTulane University School of Medicine1430 Tulane AveNew OrleansLAUnited States2020-01-092020-01-09201514243903https://doi.org/10.1016/j.pan.2014.10.008PubMedID25464937https://t.ly/1VVKEScopusMSA Google ScholarBackground Despite the incidence rate of pancreatic cancer (PC) is uncommon in developing countries, it is considered as one of the most lethal disease. Improving patients' survival requires diagnosis of the disease at early stage. Therefore, it is imperative to identify more specific and sensitive marker(s) to be used for early detection of PC. Objectives Our aim is to evaluate the potential role of circulating ADH and MIC-1 to be used as diagnostic markers in Egyptian patients and assess their value either alone or combined with CA19-9 in early detection of PC. Methods Alcohol dehydrogenase (ADH), macrophage inhibitory cytokine (MIC-1) and CA19-9 were measured by ELISA in serum procured from PC patients (n = 50) versus normal subjects (n = 20). Results Our results demonstrate that the circulating levels of ADH, MIC-1 and CA19-9 in blood of PC were significantly higher than in healthy controls (HCs) (p < 0.001). The highest marker sensitivity observed at early stage was MIC-1 (90%) and specificity was ADH (83%). The level of all three markers was elevated significantly in early stage of PC in comparison to HCs. The addition of ADH and MIC-1 to CA19-9 significantly improved the efficacy of diagnosis (p = 0.023). Conclusion Our data demonstrate that not only the combination of ADH and MIC-1 to CA19-9 can be used in early detection of PC but also can improve the overall quality of diagnosis of this lethal disease. � 2014 IAP and EPC.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityADH MIC-1CA19-9Diagnostic markersPancreatic cancerSpecificity and sensitivityalanine aminotransferasealbuminalcohol dehydrogenaseaspartate aminotransferasebilirubin glucuronideCA 19-9 antigengamma glutamyltransferaseglucosegrowth differentiation factor 15tumor markervasopressinalcohol dehydrogenaseCA 19-9 antigenGDF15 protein, humangrowth differentiation factor 15tumor markeradultagedArticleblood analysiscancer diagnosiscancer growthcancer stagingclinical articlecontrolled studycross-sectional studydiagnostic accuracydiagnostic test accuracy studydiagnostic valueearly diagnosisEgyptianenzyme activityenzyme linked immunosorbent assayfemalefollow upglucose blood levelgold standardhumaninternational normalized ratiomalepancreas cancerpredictive valuepriority journalprotein blood levelprothrombin timesensitivity and specificityvasopressin blood levelbloodcase control studyclinical trialEgyptmiddle agedPancreatic Neoplasmsvery elderlyAgedAged, 80 and overAlcohol DehydrogenaseCA-19-9 AntigenCase-Control StudiesCross-Sectional StudiesEgyptEnzyme-Linked Immunosorbent AssayFemaleGrowth Differentiation Factor 15HumansMaleMiddle AgedPancreatic NeoplasmsSensitivity and SpecificityTumor Markers, BiologicalArticlehttps://doi.org/10.1016/j.pan.2014.10.008PubMedID25464937