Waly N.E.Refaiy A.Aborehab N.M.Department of PhysiologyFaculty of MedicineHelwan UniversityCairo11795Egypt; Department of Pathology Faculty of MedicineAssiut UniversityAssiut71515Egypt; Department of BiochemistryFaculty of PharmacyMSA UniversityGiza11787Egypt2020-01-092020-01-0920179284680https://doi.org/10.1016/j.pathophys.2017.02.005PubMed ID :https://t.ly/kN7n3ScopusObjective Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor ?-1 (TGF ?-1). Methods Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2�mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats. GlcN (50 or 100�mg/kg/day, p.o. for 2 month) was administered orally. Serum levels of Il-10 and TGF-?1 were determined by ELISA. Histopathological changes in treated and control joints were examined using hematoxylin-eosin (H & E) staining. Results The mean serum level of IL-10 significantly decreased in the OA group compared to control group (P value�<�0.0001). On the other hand, mean serum level of IL-10 significantly increased in GlcN treated groups when compared to the OA group (P value�<�0.0001). Serum level of TGF ?-1 was significantly elevated in OA group compared to control group (P value�<�0.0001). On the other hand, the mean serum level of TGF ?-1 was significantly decreased in the GlcN treated groups when compared to the OA group (P value�<�0.0001). Histopathological evaluation of GlcN treated groups showed different grades of healing, according to Osteoarthritis Research Society International (OARSI) grading system. Conclusion Our results showed that IL-10 and TGF-?1 possibly mediate GlcN chondroprotective effects in OA. Both serum biomarkers can be useful in the follow-up of articular cartilage damage in clinical settings. � 2017 Elsevier B.V.EnglishGlucosamineIL-10�TGF-?Osteoarthritisbiological markerglucosamineinterleukin 10transforming growth factor betaanimal experimentanimal modelArticlechondroprotectioncontrolled studydrug mechanismenzyme linked immunosorbent assayexperimental osteoarthritishealinghistopathologymalenonhumanprotein blood levelratArticlehttps://doi.org/10.1016/j.pathophys.2017.02.005PubMed ID :