Hany Riad, AhmedAdel Hassan, MahmoudAsssem, PassantAyman, Omnia2020-12-202020-12-202020Copyright © 2020 MSA University. All Rights Reserved.http://repository.msa.edu.eg/xmlui/handle/123456789/4261A project Submitted in Partial Fulfillment of B.Sc. Degree in Pharmaceutical Sciences for October University for Modern Sciences and Arts.The human skin works as a shield against all the extrinsic factors that may harm human. It consists of many layers, and has many functions in the human. It maintains the normal internal composition of the deep layers and the external physical appearance; however, skin is affected by multiple intrinsic and extrinsic factors that may alter its composition and external appearance. Skin aging is a complex process that occurs due to multiple factors, including the aging itself, smoking, repeated facial expressions, and exposure to UV light (photoaging). The increase of an aging population and sun exposure as well as the growing interest in external appearance lead to seeking treatment for prevention or reversal of this process. Platelets rich plasma has been one safe treatment to be used to prevent skin aging or slow down the aging process. However, the current procedure is a bit painful due to injection of platelet rich plasma directly into the skin, by syringes or dermaroller. So, the current study is conducted to make a safe, pain free, hydrogel effective PRP topical formulation. Also, the penetration of PRP may be enhanced by applying physical enhancers like sonoporation and chemical enhancers like trypsin to increase penetration and give an effective treatment in a pain free PRP hydrogel formulation to be used topically on the skin. The PRP was isolated after the blood was withdrawn and centrifuged, make the gel by using Carbopol 934, prepare buffer solution and trypsin solutions with the preparation of PRP gel. Experiments are done by the physical enhancer by using the ultrasound waves and chemical enhancer by using trypsin to measure their effects on the penetration of the PRP through the skin which shows the physical enhancer has the best results on the penetration of the PRP.enMSA UniversityDSpace EgyptUniversity of Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابPharmaceuticshuman skinOther