Motawi T.Salman T.Shaker O.Abdelhamid A.Faculty of PharmacyCairo UniversityCairoEgypt; Faculty of PharmacyAl Azhar UniversityCairoEgypt; Faculty of MedicineCairo UniversityCairoEgypt; Faculty of PharmacyMSA UniversityGiza201Egypt2020-01-092020-01-09201517341922https://doi.org/10.5114/aoms.2015.54848PubMed ID :https://t.ly/gdlL6ScopusIntroduction: Adiponectin is an adipose tissue-specific protein with insulin- sensitizing properties. Many investigators have explored the association between adiponectin single nucleotide polymorphisms (SNPs) and type 2 diabetes mellitus (T2DM) in different ethnic populations from different regions. Leptin is a protein hormone constituting an important signal in the regulation of adipose tissue mass and body weight. The aim of this study was to explore potential associations between SNP +45 T>G of the adiponectin gene and SNP 2548G/A of leptin with T2DM and the effect of SNPs on serum adiponectin and leptin levels. Material and methods: From the Egyptian population, we enrolled 110 T2DM patients and 90 non-diabetic controls. Serum lipid profile, blood glucose, serum adiponectin, and leptin were measured. Genotyping for two common SNPs of the adiponectin and leptin genes was performed by polymerase chain reaction-restriction fragment length polymorphism. Results: The G allele and TG/GG genotype of SNP 45 occurred more frequently than the T allele and TT genotype in T2DM patients compares to the controls. Subjects with the GG + TG genotype of SNP 45 were at increased risk for T2DM (OR = 6.476; 95% CI: 3.401-12.33) and associated with a low serum adiponectin level compared with the TT genotype. The serum leptin concentration of GA + AA genotype carriers was not significantly different from that of the GG genotype in the diabetic group. Conclusions: The G allele carriers who have reduced plasma concentrations of adiponectin may have an association with T2DM, while leptin SNP 2548 G/A is not associated with the risk of development of T2DM in the Egyptian population. Copyright 2015 Termedia & Banach.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابPolymerase chain reaction-restriction fragment length polymorphismSingle nucleotide polymorphismadiponectinglucoseleptinlipidadultalleleArticlecontrolled studydiabetic patientEgyptiangenetic associationgenotypeglucose blood levelhumanlipid blood levelmajor clinical studymalenon insulin dependent diabetes mellituspolymerase chain reactionprotein blood levelrestriction fragment length polymorphismrisk assessmentsingle nucleotide polymorphismArticlehttps://doi.org/10.5114/aoms.2015.54848PubMed ID :