Sakr T.M.Khowessah O.M.Motaleb M.A.Abd El-Bary A.El-Kolaly M.T.Swidan M.M.Radioactive Isotopes and Generator DepartmentHot Labs CenterEgyptian Atomic Energy AuthorityPO13759CairoEgypt; Pharmaceutical Chemistry DepartmentFaculty of PharmacyModern Sciences and Arts University6th October CityEgypt; Pharmaceutics and Industrial Pharmacy DepartmentFaculty of PharmacyCairo UniversityPO11562CairoEgypt; Labeled Compounds DepartmentHot Labs CenterEgyptian Atomic Energy AuthorityPO13759CairoEgypt2020-01-092020-01-0920189280987https://doi.org/10.1016/j.ejps.2018.06.029PubMed ID 29981892https://t.ly/j67nmScopusMSA Google ScholarNanotechnology may be applied in medicine where the utilization of nanoparticles (?100 nm) for the delivery and targeting of theranostic agents is at the forefront of projects in cancer nano-science. This study points a novel one step synthesis approach to build up polyethylene glycol capped silver nanoparticles doped with I-131 radionuclide (131I-doped Ag-PEG NPs). The formula was prepared with average hydrodynamic size 21 nm, zeta potential � 25 mV, radiolabeling yield 98 � 0.76%, and showed good in-vitro stability in saline and mice serum. The in-vitro cytotoxicity study of cold Ag-PEG NPs formula as a drug carrier vehicle showed no cytotoxic effect on normal cells (WI-38 cells) at a concentration below 3 ?L/104 cells. The in-vivo biodistribution pattern of 131I-doped Ag-PEG NPs in solid tumor bearing mice showed high radioactivity accumulation in tumor tissues with maximum uptake of 35.43 � 1.12 and 63.8 � 1.3% ID/g at 60 and 15 min post intravenous (I.V.) and intratumoral injection (I.T.), respectively. Great potential of T/NT ratios were obtained throughout the experimental time points with maximum ratios 45.23 � 0.65 and 92.46 � 1.02 at 60 and 15 min post I.V. and I.T. injection, respectively. Thus, 131I-doped Ag-PEG NPs formulation could be displayed as a great potential tumor nano-sized theranostic probe. � 2018 Elsevier B.V.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابAnd nano-sized radiopharmaceuticalChelator free radiolabelingRadiochemical dopingSilver nanoparticlesTheranosticsTumor deliveryiodine 131macrogolsilver nanoparticleIodine-131metal nanoparticleradioactive iodinesilveranimal cellanimal experimentanimal modelanimal tissueArticlecancer chemotherapycell viabilitycontrolled studycytotoxicitydopingdrug delivery systemFourier transform infrared spectroscopyhumanhuman cellin vitro studyin vivo studyisotope labelingmacrophagemalemousenanoencapsulationnanotechnologynonhumanphoton correlation spectroscopypriority journalsingle drug dosesolid malignant neoplasmsurface plasmon resonancetheranostic nanomedicinetransmission electron microscopyultraviolet visible spectroscopyWI-38 cell linezeta potentialanimalcell linecell survivalchemistrydrug effectdrug releasedrug stabilitymetabolismsarcomatheranostic nanomedicinetissue distributionAnimalsCell LineCell SurvivalDrug Delivery SystemsDrug LiberationDrug StabilityHumansIodine RadioisotopesMaleMetal NanoparticlesMiceSarcomaSilverTheranostic NanomedicineTissue DistributionArticlehttps://doi.org/10.1016/j.ejps.2018.06.029PubMed ID 29981892