Moustafa A.A.Salem H.Hegazy M.Ali O.Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini StreetCairo11562Egypt; Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)6th October City11787Egypt; Analytical Chemistry DepartmentFaculty of PharmacyDeraya UniversityCairo14511Egypt2020-01-092020-01-09201513861425https://doi.org/10.1016/j.saa.2014.09.055PubMed ID 25306132https://t.ly/0E3ZxScopusSimple, accurate, and selective methods have been developed and validated for simultaneous determination of a ternary mixture of Chlorpheniramine maleate (CPM), Pseudoephedrine HCl (PSE) and Ibuprofen (IBF), in tablet dosage form. Four univariate methods manipulating ratio spectra were applied, method A is the double divisor-ratio difference spectrophotometric method (DD-RD). Method B is double divisor-derivative ratio spectrophotometric method (DD-RD). Method C is derivative ratio spectrum-zero crossing method (DRZC), while method D is mean centering of ratio spectra (MCR). Two multivariate methods were also developed and validated, methods E and F are Principal Component Regression (PCR) and Partial Least Squares (PLSs). The proposed methods have the advantage of simultaneous determination of the mentioned drugs without prior separation steps. They were successfully applied to laboratory-prepared mixtures and to commercial pharmaceutical preparation without any interference from additives. The proposed methods were validated according to the ICH guidelines. The obtained results were statistically compared with the official methods where no significant difference was observed regarding both accuracy and precision. � 2014 Elsevier B.V. All rights reserved.EnglishChlorpheniramine maleateIbuprofenMultivariate methodsPseudoephedrine HClRatio spectraUnivariate methodsChlorine compoundsDrug productsMixturesPrincipal component analysisSpectrophotometersSpectrophotometryChlorpheniramine maleateIbuprofenMultivariate methodsPseudoephedrineRatio spectraUnivariate methodLeast squares approximationschlorpheniraminedecongestive agentdrug combinationhistamine H1 receptor antagonistibuprofennonsteroid antiinflammatory agentpseudoephedrinetabletcommon coldcomparative studydrug combinationevaluation studylimit of detectionmultivariate analysisproceduresregression analysisspectrophotometrytabletAnti-Inflammatory Agents, Non-SteroidalChlorpheniramineCommon ColdDrug CombinationsHistamine H1 AntagonistsIbuprofenLeast-Squares AnalysisLimit of DetectionMultivariate AnalysisNasal DecongestantsPseudoephedrineSpectrophotometryTabletsArticlehttps://doi.org/10.1016/j.saa.2014.09.055PubMed ID 25306132