Saleh S.S.Lotfy H.M.Hassan N.Y.Salem H.Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)11787 6th October CityEgypt; Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini Street11562 CairoEgypt2020-01-092020-01-09201413861425https://doi.org/10.1016/j.saa.2014.05.004PubMed ID 24873889https://t.ly/ndveYScopusMSA Google ScholarThis work represents a comparative study of a novel progressive spectrophotometric resolution technique namely, amplitude center method (ACM), versus the well-established successive spectrophotometric resolution techniques namely; successive derivative subtraction (SDS); successive derivative of ratio spectra (SDR) and mean centering of ratio spectra (MCR). All the proposed spectrophotometric techniques consist of several consecutive steps utilizing ratio and/or derivative spectra. The novel amplitude center method (ACM) can be used for the determination of ternary mixtures using single divisor where the concentrations of the components are determined through progressive manipulation performed on the same ratio spectrum. Those methods were applied for the analysis of the ternary mixture of chloramphenicol (CHL), dexamethasone sodium phosphate (DXM) and tetryzoline hydrochloride (TZH) in eye drops in the presence of benzalkonium chloride as a preservative. The proposed methods were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulation containing the cited drugs. The proposed methods were validated according to the ICH guidelines. A comparative study was conducted between those methods regarding simplicity, limitation and sensitivity. The obtained results were statistically compared with those obtained from the official BP methods, showing no significant difference with respect to accuracy and precision. � 2014 Elsevier B.V. All rights reserved.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityAmplitude center methodChloramphenicolDexamethasoneSuccessive derivative ratioSuccessive derivative subtractionTetryzolineAntibioticsChlorine compoundsSpectrophotometersSpectrophotometryAmplitude center methodChloramphenicolDexamethasonesSuccessive derivativesTetryzolineMixtureschloramphenicoldexamethasoneimidazole derivativetetryzolinechemistrycomparative studydrug formulationproceduresreproducibilityspectrophotometrystandardChloramphenicolDexamethasoneDrug CompoundingImidazolesReference StandardsReproducibility of ResultsSpectrophotometryArticlehttps://doi.org/10.1016/j.saa.2014.05.004PubMed ID 24873889