Bakr R.O.Mohamed S.A.E.H.Ayoub N.Department of PharmacognosyFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)Egypt; Department of PharmacognosyFaculty of PharmacyAl-Azhar University (Girls)Egypt; Department of PharmacognosyFaculty of PharmacyBritish University in Egypt (BUE)Egypt2020-01-092020-01-0920161896016https://doi.org/10.21010/ajtcam.v13i6.19PubMed ID 28480370https://t.ly/7OJ7XScopusBackground: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). Material and Methods: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. Results: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC50of 12.1, 30.9, 11.7 and 19.5 ?g/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). Conclusion: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent. 2016, African Ethnomedicines Network. All rights reserved.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityCentaurea aegyptiacaCytotoxicityFlavonoidMTT assay3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromideantineoplastic agentantivirus agentCentaurea aegyptiaca extractCentaurea aegyptiaca plant extractdoxorubicinflavone derivativeflavonolmethanolphenolplant extractunclassified drugantineoplastic agentantivirus agentphenol derivativeplant extractantineoplastic activityantiviral activityArticlecancer cell lineCentaureaCentaurea aegyptiacachemical compositioncollisionally activated dissociationcontrolled studydrug mechanismEgyptelectrospray mass spectrometryHCT 116 cell lineHeLa cell lineHep-G2 cell lineHepatitis A virusHerpes simplex virus 1humanhuman cellIC50liquid chromatographyMCF-7 cell lineMTT assaynonhumanphytochemistryaerial plant partchemistrytandem mass spectrometryAntineoplastic Agents, PhytogenicAntiviral AgentsCentaureaEgyptHCT116 CellsHeLa CellsHep G2 CellsHumansMCF-7 CellsPhenolsPlant Components, AerialPlant ExtractsTandem Mass SpectrometryArticlehttps://doi.org/10.21010/ajtcam.v13i6.19PubMed ID 28480370