Khattab F.I.Salem H.Riad S.M.Elbalkiny H.T.Cairo UniversityAnalytical Chemistry DepartmentFaculty of PharmacyKasr-El Aini Street11562 CairoEgypt; October University for Modern Sciences and Arts (MSA)Analytical Chemistry DepartmentFaculty of Pharmacy11787 6th October CityEgypt2020-01-092020-01-0920149334173https://doi.org/10.1556/JPC.27.2014.4.9PubMed ID :https://t.ly/DXEx2ScopusTwo methods were described for the simultaneous determination of ciprofloxacin HCl (CIP) and moxifloxacin HCl (MOX) in their binary mixture present in industrial wastewater. A solid-phase extraction procedure (SPE) based on retention on HLB OASIS cartridges and elution with a mixture of methanol-water in acidic medium was preformed, and then both fluoroquinolones were separated using two chromatographic methods. The first method was based on high-performance liquid chromatographic separation of the two drugs on reversed-phase Zorbax C18 column. The mobile phase consisted of monobasic potassium phosphate (50 mM, pH 2.5, adjusted with phosphoric acid) and acetonitrile (80:20, v/v). Flow rate was 1 mL min-1. Quantitation was achieved with ultraviolet (UV) detection at 278 nm. Linearity was found to be over the concentration range of 1-50 ?g mL-1 for both CIP and MOX. The second method was based on the thin-layer chromatographic (TLC) separation of the two drugs followed by densitometric measurements of their bands at 278 nm. The separation was carried out on silica gel 60 F254 plates, using methanol, ammonia, and methylene chloride (55:35:20, v/v) as a developing system. The linearity was found to be in the range of 0.25-2.5 ?g band -1 for both CIP and MOX. Both methods were optimized and validated as per International Conference on Harmonization (ICH) guidelines. Separation was developed on spiked water samples and checked on process wastewaters of industrial origin after SPE sample pretreatment.EnglishCiprofloxacin hydrochlorideHigh-performance liquid chromatographyIndustrial wastewaterMoxifloxacin hydrochlorideThin-layer chromatography-densitometryAntibioticsBinary mixturesChromatographic analysisDichloromethaneHigh performance liquid chromatographyHigh pressure liquid chromatographyMethanolSeparationSilica gelChromatography-densitometryCiprofloxacin hydrochlorideFluoroquinolone antibioticsIndustrial wastewatersInternational conference on harmonizationsLiquid chromatographic separationsMoxifloxacinSimultaneous determinationsChromatographyArticlehttps://doi.org/10.1556/JPC.27.2014.4.9PubMed ID :