Morsi N.M.Aboelwafa A.A.Dawoud M.H.S.Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyCairo UniversityCairoEgypt; Department of PharmaceuticsFaculty of PharmacyModern Sciences and Arts UniversityCairoEgypt2020-01-092020-01-09201620901232https://doi.org/10.1016/j.jare.2016.07.003PubMed ID :https://t.ly/7OGDXScopusMSA Google ScholarTimolol maleate (TiM), a nonselective ?-adrenergic blocker, is a potent highly effective agent for management of hypertension. The drug suffers from extensive first pass effect, resulting in a reduction of oral bioavailability (F%) to 50% and a short elimination half-life of 4 h; parameters necessitating its frequent administration. The current study was therefore, designed to formulate and optimize the transfersomal TiM gel for transdermal delivery. TiM loaded transfersomal gel was optimized using two 23 full factorial designs; where the effects of egg phosphatidyl choline (PC): surfactant (SAA) molar ratio, solvent volumetric ratio, and the drug amount were evaluated. The formulation variables; including particle size, drug entrapment efficiency (%EE), and release rate were characterized. The optimized transfersomal gel was prepared with 4.65:1 PC:SAA molar ratio, 3:1 solvent volumetric ratio, and 13 mg drug amount with particle size of 2.722 ?m, %EE of 39.96%, and a release rate of 134.49 ?g/cm2/h. The permeation rate of the optimized formulation through the rat skin was excellent (151.53 ?g/cm2/h) and showed four times increase in relative bioavailability with prolonged plasma profile up to 72 h compared with oral aqueous solution. In conclusion, a potential transfersomal transdermal system was successfully developed and the factorial design was found to be a smart tool, when optimized. � 2016EnglishAntihypertensiveFactorial designOptimizationTimolol maleateTransdermalTransfersomesArticlehttps://doi.org/10.1016/j.jare.2016.07.003PubMed ID :