Abdel Hakim Mousa, AmanyMohamed Naguib Abdel Moawed, DenaMohamed Abdallah Elberry, Adel2020-06-272020-06-272020-04. Alshamali F, Alkhayat AQ, Budowleb B, et al. 2004. Y chromosome in forensic casework and paternity testing. International Congress Series. 1261: 353-356. Ref.: https://bit.ly/2VLleVd 13. Burgarella C, Navascues M. 2010. Mutation rate estimates for 110 Y-chromosome STRs combining population and father-son pair data. Eur J Hum Genet. 19: 70-75. Ref.: https://bit.ly/3avZnXq 14.Vanek D, Saskova L, Koch H. 2009. Kinship and Y-chromosome analysis of 7th century human remains: novel DNA extraction and typing procedure for ancient material. Croat Med J. 50: 286-295. Ref.: https://bit.ly/3eGefW8 15. Ge J, Eisenberg A, Budowle B. 2012. Developing criteria and data to determine best options for expanding the core CODIS loci. Investig Genet. 3: 1. Ref.: https://bit.ly/2yvocVU 16. Ge J, Sun H, Li H, et al. 2014. Future directions of forensic DNA databases. Croat Med J. 55:163-166. Ref.: https://bit.ly/3aqhVrU Okamoto O, Yamamoto Y, Inagaki S, et al. 2003. Analysis of Short Tandem Repeat (STR) Polym orphisms by the PowerPlex 16 Systemhttps://doi.org/10.36811/ijfs.2020.110012https://t.ly/AVQ1MSA GOOGLE SCHOLARBackground: In forensic field, for establishing the paternity of disputed offspring, STR typing (autosomal, and Y typing) are commonly used to dissolve the cases. Short tandem repeat (STR) technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another. The Federal Bureau of Investigation (FBI) uses a standard set of 13 specific STR regions for CODIS. Aim of the study: The present work was done to find out the biological father of child in a case where mother was killed by her husband for the doubt of her pregnancy. Methods: After taking blood samples from the murdered mother, the baby and the killer husband, Autosomal STRs Using AmpFlSTR Identifiler Plus PCR Amplification Kit and Y-STR analysis using AmpFlSTR Yfiler PCR Amplification Kit were done to solve the disputed paternity case. Results: After the analysis of DNA STRs profiles of autosomal and Y-chromosome markers, the dead fetus was proved to be the son of the killer father. Conclusions: Autosomal STR multiplex analysis combined with Y-STR analysis help to reach a conclusion in disputed paternity cases with greater confidence.en-USDisputedPaternityMultiplexShort tandem repratsY-STRAnalysisArticlehttps://doi.org/10.36811/ijfs.2020.110012