Hamed, Ahmed GamalMohammed, Mariam HaniRezk, Mohamed AshrafHelmi, Rana Ahmed2024-06-042024-06-042023Faculty Of Pharmacy Graduation Project 2022 - 2023http://repository.msa.edu.eg/xmlui/handle/123456789/6007Aim Poultry are great sources of protein and other important nutrients for our body. So, poultry should be included as a part of a balanced diet. Residues of veterinary drugs in poultry meat have serious health effects on humans (e.g., antimicrobial resistance, carcinogenicity, and hypersensitivity), making controlling veterinary drug residues an important parameter in ensuring consumer protection. Hence, it is important to study the withdrawal time of these drugs in chicken tissues. Materials and Methods This work was performed to quantitatively determine a co-formulated veterinary formulation namely, Coccimix®, consisting of two sulfonamides (Sulfadimidine sodium and Sulfaquinoxaline sodium), in addition to Diaveridine, and Vitamin K3 in different tissues of broiler chickens including muscles (breast and thigh) and liver. The chickens were treated with the recommended dose of Coccimix® formulation. The analysis was done by solvent extraction for clean-up of samples from the tissue matrix, followed by HPLC-MS/MS method using Agilent C18 4.6*50 mm 2.7-Micron as a stationary phase and a mobile phase of acetonitrile: 0.1% formic acid in water (85: 15 v/v) with positive electrospray ionization. Results The proposed method was validated according to FDA guidance for bioanalytical method validation. The residues of the four components declined to reach levels lower than MRLs over a period of 7 days. Both sulfonamides and Diaveridine were found to be more persistent in liver tissues than muscle tissues. Conclusion It is recommended to monitor the withdrawal (elimination), especially for antibiotics, before marketing to ensure the hygienic suitability of broilers edibles for safe human consumptionen-USOctober University For Modern Sciences and ArtsOctober University For Modern Sciences and Arts MSAMSAجامعة أكتوبر للعلوم الحديثة والأدابDRUGSTISSUESOther