Aborehab N.M.El Bishbishy M.H.Refaiy A.Waly N.E.MSA UniversityDepartment of BiochemistryFaculty of PharmacyGiza11787Egypt; MSA UniversityDepartment of PharmacognosyFaculty of PharmacyGiza11787Egypt; Assiut UniversityDepartment of Pathology Faculty of MedicineAssiut71515Egypt; Department of PhysiologyFaculty of MedicineHelwan UniversityCairo11795Egypt2020-01-092020-01-09201714726882https://doi.org/10.1186/s12906-017-2002-yPubMed ID 29166937https://t.ly/6xMX1ScopusBackground: Herbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model. Methods: Thirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1?) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA. Results: Serum levels of COMP, HA, MPO, MDA, and IL-1? were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001). Conclusions: The ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect. � 2017 The Author(s).EnglishCOMPCurcuminGingerHAIL-1?MDAMPOOsteoarthritisalcoholcartilage oligomeric matrix proteincurcuminginger extracthyaluronic acidinterleukin 1betaiodoacetic acidmalonaldehydemyeloperoxidasesuperoxide dismutasecartilage oligomeric matrix proteincurcuminhyaluronic acidinterleukin 1betamalonaldehydeperoxidaseplant extractprotective agentsuperoxide dismutaseanimal experimentanimal modelanimal tissueantiinflammatory activityantioxidant activityArticlechondroprotectioncontrolled studydrug effectdrug mechanismEgyptenzyme activityenzyme linked immunosorbent assayhistopathologyliquid chromatography-mass spectrometrymalenonhumanosteoarthritisprotein blood levelrandomized controlled trialratanimalbloodchemistrydisease modelgingermetabolismAnimalsCartilage Oligomeric Matrix ProteinCurcuminDisease Models, AnimalGingerHyaluronic AcidInterleukin-1betaMaleMalondialdehydeOsteoarthritisPeroxidasePlant ExtractsProtective AgentsSuperoxide DismutaseArticlehttps://doi.org/10.1186/s12906-017-2002-yPubMed ID 29166937