Ezzat, Shahira MEzzat M.I.Okba M.M.Hassan S.M.Alkorashy A.I.Karar M.M.Ahmed S.H.Mohamed S.O.Fernandes G.Pharmacognosy DepartmentFaculty of PharmacyCairo UniversityKasr El-Ainy StreetCairo11562Egypt; Pharmacognosy DepartmentFaculty of PharmacyOctober University for Modern Science and Arts (MSA)6th of October 12566Egypt; Department of BiochemistryFaculty of ScienceAin Shams UniversityCairoEgypt; Department of BiochemistryFaculty of PharmacyAl-Azhar UniversityCairoEgypt; Zewail City of Science and TechnologyCairoEgypt; Department of BiochemistryFaculty of AgricultureCairo UniversityCairoEgypt; School of Pharmaceutical SciencesUniversiti Sains MalaysiaMalaysia2020-01-092020-01-0920191741427Xhttps://doi.org/10.1155/2019/7543460PubMed ID :https://t.ly/9LLB3ScopusMSA Google ScholarEurycoma longifolia Jack (Fam.: Simaroubaceae), known as Tongkat Ali (TA), has been known as a symbol of virility and sexual power for men. Metabolic profiling of the aqueous extract of E. longifolia (AEEL) using UPLC-MS/MS in both positive and negative modes allowed the identification of seventeen metabolites. The identified compounds were classified into four groups: quassinoids, alkaloids, triterpenes, and biphenylneolignans. AEEL is considered safe with oral LD50 cut-off >5000 mg/kg. Oral administration of 50, 100, 200, 400, or 800 mg/kg of AEEL for 10 consecutive days to Sprague-Dawley male rats caused significant reductions in mounting, intromission, and ejaculation latencies and increased penile erection index. AEEL increased total body weight and relative weights of seminal vesicles and prostate. Total and free serum testosterone and brain cortical and hippocampal dopamine content was significantly elevated in treated groups with no significant effects on serotonin or noradrenaline content. 2019 Shahira M. Ezzat et al.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابdopamineEurycoma longifolia extractfollitropinluteinizing hormonenoradrenalinquassinoid derivativeserotonintestosteroneaphrodisiac activityArticlebody weightbrain cortexchemical compositioncontrolled studydrug activityejaculationEurycoma longifoliafemalefollitropin blood levelhippocampusionizationlatent periodLD50luteinizing hormone blood levelmalemount latencynonhumannoradrenalin levelpenis erectionprostate weightratseminal vesiclesexual behaviorSimaroubaceaesingle drug dosetandem mass spectrometrytestosterone blood levelultra performance liquid chromatographyArticlehttps://doi.org/10.1155/2019/7543460PubMed ID :