Ezzat, Shahira MA. Choucry, MouchiraA. Kandil, Zeinab2019-11-042019-11-042015Aliyeva E, Umur S, Zafer E, Acigoz G. (2004). The effect of polylactide membranes on the levels of reactive oxygen species in periodontal flaps during wound healing. Biomaterials 25:4633–7. Anandjiwala S, Srinivasa H, Kalola J, Rajani M. (2007a). Free-radical scavenging activity of Bergia suffruticosa (Delile) Fenzl. Nat Med 6: 59–62. Anandjiwala S, Srinivasa H, Rajani M. (2007b). Isolation and TLC Densitometric quantification of gallicin, gallic acid, lupeol and b-sitosterol from Bergia suffruticosa, a hitherto unexpected plant. Chromatographia 66:725–34. Atta AH, Alkohafi A. (1998). Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plants extracts. J Ethnopharmacol 60:117–24. Baboir BM. (1978). Oxygen dependent microbial killing by phagocytes (first of two parts). N Eng J Med 298:629–68. British Pharmacopoiea. (1996). Simple Ointment. London: HMSO, 1360. Buffoni F, Bancheli G, Cambi S, et al. (1993). Skin wound healing: Some biochemical parameters in Guinea pig. J Pharmaceutics Pharmacol 45:784–90. Clark RAF. (1996). Wound repair: Overview and general consideration. In: Clark RA, Henson PM, eds. Molecular and Cellular Biology of Wound Repair. New York: The Plenum Press, 112–13. Davis CC, Chase MW. (2004). Elatinaceae are sister to Malpighiaceae; Peridiscaceae belong to Saxifragales. Am J Bot 91:262–73. Delazar A, Byres M, Gibbons S, et al. (2004). Iridoid glycosides from Eremostachys glabra. J Nat Prod 67:1584–7. Elizabeth K, Rao MNA. (1990). Oxygen radical scavenging activity of curcumin. Int J Pharm 58:237–40. Fleischer AB, Feldman SR, White RE, et al. (1997). Procedures for skin diseases performed by physicians in 1993 and 1994: Analysis of data from the national ambulatory medical care survey. J Am Acad Dermatol 37:719–24. Glynn LE. (1981). The pathology of scar tissue formation. In: Glynn LE, ed. Handbook of Inflammation, vol. 3. Tissue Repair and Regeneration. Amsterdam: Elsevier/North Holland Biomedical Press, 34–5. Goad J, Akihisa, T. (1997). Analysis of Sterols, 1st edn. London: Blackie Academic and Professional Press, Champan and Hall. Gomathi K, Gopinath D, Rafiuddin AM, Jayakumar R. (2003). Quercetin incorporated collagen matrices for dermal wound healing processes in rats. Biomaterials 24:2767–72. Heinrich M, Gibbons S. (2001). Ethnopharmacology in drug discovery: An analysis of its role and potential contribution. J Pharmacy Pharmacol 53:425–32. Hoskeri YH, Krishna V, Jignesh S, et al. (2012). In-silico drug designing using b-sitosterol isolated from Flaveria trinervia against peptide deformylase protein to hypothesize bactericidal effect. Int J Pharmacy Pharm Sci 4:192–6. Jewo PI, Fadeyibi IO, Babalola OS, et al. (2009). A comparative study of the wound healing properties of moist exposed burn ointment (Mebo) and silver sulphadiazine. Ann Burns Fire Disasters XXII: 79–82. Kumar B, Vijayakumar M, Govindarajan R, Pushpangadan P. (2007). Ethnopharmacological approaches to wound healing – Exploring medicinal plants of India. J Ethnopharmacol 114:103–13. Mabry TJ, Markham KR, Thomas MB. (1970). The Systematic Identication of Flavonoids. Berlin: Springer-Verlag. Manivannan R, Prabakaran K, Ilayaraja S. (2014). Isolation, identification and antibacterial and wound healing studies of quercetin-3-o-a-Lrhamnopyranoside-200-gallate. Int J Appl Sci Eng 12:99–106. Markham KR. (1982). Techniques of Flavonoid Identification. London: Academic Press. Majumdar MR, Kamath JV. (2005). Herbal concept on wound healing. J Pharmaceut Res 4:01–07. Narendhirakannan RT, Nirmala JG, Caroline A, et al. (2012). Evaluation of antibacterial, antioxidant and wound healing properties of seven traditional medicinal plants from India in experimental animals. Asian Pac J Trop Biomed 2:S1245–53. NCCLS (National Committee for Clinical Laboratory Standards). (2000). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically – 5th edn. Approved Standard M7-A5. Wayne (PA): NCCLS. Ozgen M, Reese RN, Tulio AZ, et al. (2006). Modified 2,2-azino-bis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,20 -diphenyl-1- picrylhydrazyl (DPPH) methods. J Agric Food Chem 54:1151–7. Okoli CO, Akah PA, Ezugworie U. (2006). Anti-inflammatory activity of extracts of root bark of Securidaca longipedunculata Fres (Polygalaceae). Afr J Trad Compl Alter Med 3:54–63. O¨ ksu¨z S, Ulubelen A, Barla A. (2002). Terpenoids and aromatic compounds from Euphorbia heteradena. Turk J Chem 26:457–63. Parekh J, Nair R, Chanda S. (2005). Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Ind J Pharmacol 37:408–9. Saleem M. (2009). Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett 285:109–15. Takao T, Watanabe N, Yagi I, Sakata K. (1994). A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci Biotech Biochem 58: 1780–3. Thiem B, Grosslinka O. (2003). Antimicrobial activity of Rubus chamaemorus leaves. Fitoter 75:93–5. Ur-Rehman A, Siddiqa A, Abbasi MA, et al. (2013). Bergia ammannioides: Phytochemical screening, antioxidant activity and radical scavenging effects of its various fractions. Asian J Chem 25: 7921–6. Yan XH, Guo YW. (2004). Two newellagic acid glycosides from leaves of Diplopanax stachyaanthus. J Asian Nat Prod Res 6:271–6. Woessner JF. (1961). The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 93:440–7.1388-0209 print1744-5116 onlinehttps://doi.org/https://cutt.ly/ftwjgG7MSA Google ScholarContext: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use. Objective: The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions. Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents. Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren) and antibacterial activity (MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS+ , and super oxide radical with an IC50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside, and quercetin-3-O-a-L-rhamnoside were isolated from EtFr. Discussion and conclusion: Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from BergiaenCycloartenolcyclolaudenolwound complicationsArticlehttps://doi.org/