Aly, Shaza HElissawy, Ahmed MFayez, Ahmed MEldahshan, Omayma AElshanawany, Mohamed ASingab, Abdel Nasser B2020-07-262020-07-267/22/2020https://doi.org/10.1080/14786419.2020.1795853https://t.ly/qI4OFive flavonoids were isolated from the ethyl acetate fraction of leaves of Sophora secundiflora; formononetin (1), 5-hydroxy-4'-methoxyflavone (2), genistein (3), 5-hydroxy-8-(1-hydroxy-1-methyl-ethyl)-2-(4-hydroxyphenyl)-4H-furo-[2, 3-h]-chromen-4-one (4) and ononin (5). Additionally, LC-ESI-MS/MS analysis of the ethyl acetate fraction of S. secundiflora leaves had led to tentative identification of eighteen compounds. Formononetin, S. tomentosa and S. secundiflora leaves methanolic extract were evaluated in vivo for their neuroprotective activity where formononetin and S. tomentosa showed promising neuroprotective activity with reduction in acetylcholine esterase (AchE) enzyme activity and elevation of acetylcholine (Ach) and glutathione(GSH) brain levels and attenuation of dopamine (DA), nor-adrenaline (NA) and malonedialdehyde (MDA) brain level significantly, However S. secundiflora leaves methanolic extract didn't attenuate the AchE enzyme activity, DA and NA brain levelsen-USscopolamine.neuroprotectiveformononetinflavonoidsLC/MSSophoraSophoraArticlehttps://doi.org/10.1080/14786419.2020.1795853