Moustafa E.S.Swilam N.F.Ghanem O.B.Hashim A.N.Nawwar M.A.Lindequist U.Linscheid M.W.October University for Modern Sciences and Arts6th October CityEgypt; Pharmacognosy DepartmentFaculty of PharmacyBritish UniversityEgypt; Under Graduate Research TraineeFaculty of PharmacyGerman UniversityCairoEgypt; Department of Phytochemistry and Plant SystematicsNational Research Center14 El Tahrier Str.DokkiCairo1361Egypt; Pharmaceutical BiologyInstitute of PharmacyErnst-Moritz-Arndt-UniversityGreifswaldGermany; Laboratory of Applied Analytical and Environmental ChemistryHumboldt-UniversityBerlinGermany2020-01-092020-01-092018317144https://doi.org/10.1691/ph.2018.7946PubMed ID 29609693https://t.ly/ZvH2ScopusPhenolic metabolite profiling using two dimensional paper chromatographic analysis (2 DPC) was used for assaying the complex mixture of phenolics of an aqueous ethanol aerial part extract of Cuphea ignea (Lytheraceae). A coumarin with a rare structure, namely, 7-hydroxy 3-methoxy coumarin 5-O-?-glucopyranoside was isolated from the investigated extract. The structure was elucidated by conventional methods and spectral analysis, including one and two dimensional NMR (1D and 2D NMR), as well as by interpretation of the spectra obtained by high resolution electrospray ionization mass technique (HRESIMS). The rare coumarin significantly inhibited reactive oxygen species production with an ED50 value of 6.31�1.64 ?g/ml and 5.78�0.66 ?g/ml as determined by the the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the oxygen radical absorption capacity (ORAC) assay respectively. The isolated coumarin presented a cytotoxic activity assessed by using the neutral red assay (NRU) against lung cancer cell line (H23) with IC50 of 40.38�2.75 ?g/ml. � 2018 Govi-Verlag Pharmazeutischer Verlag GmbH. All rights reserved.English7 hydroxy 3 methoxycoumarin 5 o beta glucopyranosidecoumarin derivativeCuphea ignea extractetoposideplant extractreactive oxygen metaboliteunclassified drug1,1-diphenyl-2-picrylhydrazylantineoplastic agentantioxidantbiphenyl derivativecoumarin derivativepicric acidplant extractreactive oxygen metabolitescavengerantioxidant activityArticlecell viabilitycontrolled studyCupheaCuphea igneaDPPH radical scavenging assaydrug cytotoxicitydrug identificationdrug isolationdrug structureED50HaCat cell lineHCT 116 cell lineHuh-7 cell linehumanhuman cellIC50NCI-H23 cell lineNCI-H460 cell lineORAC assaycell survivalchemistrydrug effectelectrospray mass spectrometryflowermetabolismnuclear magnetic resonance spectroscopytumor cell lineAntineoplastic Agents, PhytogenicAntioxidantsBiphenyl CompoundsCell Line, TumorCell SurvivalCoumarinsCupheaFlowersFree Radical ScavengersHumansMagnetic Resonance SpectroscopyPicratesPlant ExtractsReactive Oxygen SpeciesSpectrometry, Mass, Electrospray IonizationArticlehttps://doi.org/10.1691/ph.2018.7946PubMed ID 29609693