Lotfy H.M.Mohamed D.Elshahed M.S.Pharmaceutical Chemistry DepartmentFaculty of Pharmaceutical Sciences & Pharmaceutical IndustriesFuture University in EgyptCairo12311Egypt; Analytical Chemistry DepartmentFaculty of PharmacyHelwan UniversityEin HelwanCairo11795Egypt; Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts11787Egypt2020-01-092020-01-09201813861425https://doi.org/10.1016/j.saa.2017.08.015PubMed ID 28802856https://t.ly/1VJrEScopusIn the presented work several spectrophotometric methods were performed for the quantification of canagliflozin (CGZ) and metformin hydrochloride (MTF) simultaneously in their binary mixture. Two of these methods; response correlation (RC) and advanced balance point-spectrum subtraction (ABP-SS) were developed and introduced for the first time in this work, where the latter method (ABP-SS) was performed on both the zero order and the first derivative spectra of the drugs. Besides, two recently established methods; advanced amplitude modulation (AAM) and advanced absorbance subtraction (AAS) were also accomplished. All the proposed methods were validated in accordance to the ICH guidelines, where all methods were proved to be accurate and precise. Additionally, the linearity range, limit of detection and limit of quantification were determined and the selectivity was examined through the analysis of laboratory prepared mixtures and the combined dosage form of the drugs. The proposed methods were capable of determining the two drugs in the ratio present in the pharmaceutical formulation CGZ:MTF (1:17) without the requirement of any preliminary separation, further dilution or standard spiking. The results obtained by the proposed methods were in compliance with the reported chromatographic method when compared statistically, proving the absence of any significant difference in accuracy and precision between the proposed and reported methods. � 2017 Elsevier B.V.EnglishAdvanced absorbance subtraction methodAdvanced amplitude modulation methodAdvanced balance point using spectrum subtraction methodCanagliflozinMetformin hydrochlorideResponse correlation methodAmplitude modulationBinary mixturesChromatographic analysisDrug dosageMixturesModulationOptical transfer functionSpectrophotometryAdvanced amplitude modulation methodCanagliflozinMetformin hydrochloridesSpectrum subtractionsSubtraction methodDrug productsantidiabetic agentcanagliflozinmetforminchemistrydrug formulationlimit of detectionproceduresreproducibilitysolution and solubilityspectrophotometrytheoretical modelCanagliflozinDrug CompoundingHypoglycemic AgentsLimit of DetectionMetforminModels, TheoreticalReproducibility of ResultsSolutionsSpectrophotometryArticlehttps://doi.org/10.1016/j.saa.2017.08.015PubMed ID 28802856