El-Khazragy N.Ghozy S.Matbouly S.Zaki W.Safwat G.Hussien G.Khalifa O.Clinical Pathology and Haematology DepartmentFaculty of MedicineAin Shams University Biomedical Research DepartmentCairoEgypt; Neurosurgery DepartmentFaculty of MedicineMansoura UniversityMansouraEgypt; Department of PaediatricFaculty of MedicineAin Shams UniversityCairoEgypt; Department of BiochemistryFaculty of MedicineAin Shams UniversityCairoEgypt; Department of Molecular BiologyFaculty of BiotechnologyOctober University for Modern Sciences and Arts (MSA)CairoEgypt2020-01-092020-01-0920197302312https://doi.org/10.1002/jcb.28796PubMedID31038787https://t.ly/DXGylScopusObjectives: To investigate the correlation of homeobox (HOX) transcript antisense RNA expression with clinicopathological features and the clinical prognosis of the patients with chromosome 12p abnormalities associated acute myeloid leukemia (AML). We also investigate the association of 12p chromosomal on the expression of HOTAIR, miRNA-193a, and c-kit gene as targeting genes for HOTAIR in AML. Methods: AML patients with 12p chromosomal abnormalities were recruited and compared to AML with other chromosomal abnormalities rather than 12p. The long noncoding RNA (lncRNA) �HOTAIR,� miR-193a, and c-Kit genes expression were measured in bone marrow samples using Syber green based real-time polymerase chain reaction. Results: We found a significant difference for the expression levels of HOTAIR, c-kit, and miR-193a between 12p abnormalities associated AML and those without. The survival analysis revealed that patient's with low expression levels of HOTAIR and c-kit had significantly better survival and leukemia free survival. In contrast, miR-193a was associated with better overall survival but not leukemia free survival. Conclusion: 12p abnormalities associated AML were associated with worse prognosis. Our results proved that HOTAIR, miR-193a, and c-kit genes are independent prognostic predictors in 12p chromosomal associated AML; therefore it may represent a novel therapeutic application in AML in the future. � 2019 Wiley Periodicals, Inc.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA University12p aberrations12p deletionacute myeloid leukemiac-KIThomeobox transcript antisense RNAmiR-193aanthracyclineasparaginasecomplementary DNAcomplementary RNAcorticosteroidhomeobox transcript antisense RNAlong untranslated RNAmicroRNAmicroRNA 193astem cell factor receptorunclassified drugvincristineacute myeloid leukemiaadultagedArticlebone marrowcancer prognosiscancer specific survivalchromosome 12pchromosome aberrationchromosome deletioncontrolled studyfemalegene targetinghumanhuman cellhuman tissueinduction chemotherapymajor clinical studymaleminimal residual diseaseoverall survivalpriority journalreal time polymerase chain reactionsurvival analysisArticlehttps://doi.org/10.1002/jcb.28796PubMedID31038787