Zafer M.M.Al-Agamy M.H.El-Mahallawy H.A.Amin M.A.Ashour M.S.E.-D.Department of Microbiology and ImmunologyFaculty of PharmacyAhram Canadian UniversityCairoEgypt; Department of Pharmaceutics and MicrobiologyCollege of PharmacyKing Saud UniversityP.O. Box 2457Riyadh 11451Saudi Arabia; Department of Microbiology and ImmunologyFaculty of PharmacyAl-Azhar UniversityCairoEgypt; Department of Clinical PathologyNational Cancer InstituteCairo UniversityCairoEgypt; Department of Microbiology and ImmunologyFaculty of PharmacyCairo UniversityCairoEgypt; Department of Microbiology and ImmunologyFaculty of PharmacyModern Science and Arts University6th October CityGizaEgypt2020-01-092020-01-09201423146133https://doi.org/10.1155/2014/101635PubMed ID 24707471https://t.ly/YZXevScopusThis study was designed to investigate the prevalence of metallo-?-lactamases (MBL) and extended-spectrum ?-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM2, bla OXA10-, bla VEB1, bla NDM-, and bla IMP1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM2- and bla OXA10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM2, bla IMP1, bla NDM, and bla OXA10 in P. aeruginosa in Egypt. � 2014 Mai M. Zafer et al.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابaztreonamcefoperazoneceftazidimecefuroximeextended spectrum beta lactamaseimipenemmetallo beta lactamasepiperacillin plus tazobactambacterial DNAbeta lactamaseantibiotic resistancearticlebacterial genebacterial strainbacterium isolatebacterium isolationcancer patientcontrolled studygenetic codehumanminimum inhibitory concentrationmulticenter studynonhumanphenotypepolymerase chain reactionprevalencePseudomonas aeruginosabacterial geneEgyptgeneticsmicrobiologymultidrug resistanceneoplasmPseudomonas aeruginosabeta-LactamasesDNA, BacterialDrug Resistance, Multiple, BacterialEgyptGenes, BacterialHumansNeoplasmsPseudomonas aeruginosaArticlehttps://doi.org/10.1155/2014/101635PubMed ID 24707471