Samir, AhmedSalem, HeshamAbdelkawy, Mohammad2020-03-052020-03-0520121. Sadler NP, Jacobs H (1995) Application of the Folin-Ciocalteau reagent to the determination of salbutamol in pharmaceutical preparations. Talanta 42: 1385- 1388. 2. Dalibor S, Rolf K, Antonın S (2002) Anal Chimica Acta 455: 103-109. 3. Mohamed GG, Shaban MK, Zayed MA, Abd El-Hamid EM (2002) 2,6-Dichloroquinone chlorimide and 7,7,8,8-tetracyanoquinodimethane reagents for the spectrophotometric determination of salbutamol in pure and dosage forms. J Pharma Biomed Anal 28: 1127-1133. 4. Bakry RS, Razak OA, El Walily AF, Belal SF (1996) Spectrophotometric determination of salbutamol sulphate using chlorinated quinones in the presence or absence of acetaldehyde. J Pharma Biomed Anal 14: 357-362. 5. Dol I, Knochen M (2004) Flow-injection spectrophotometric determination of salbutamol with 4-aminoantipyrine. Talanta 64: 1233-1236. 6. Dave HN, Mashru RC, Thakkar AR (2007) Simultaneous determination of salbutamol sulphate, bromhexine hydrochloride and etofylline in pharmaceutical formulations with the use of four rapid derivative spectrophotometric methods. Anal Chimica Acta 597: 113-120. 7. Lindino CA, Bulhões LO (2007) Determination of fenoterol and salbutamol in pharmaceutical formulations by electrogenerated chemiluminescence. Talanta 72: 1746-1751. 8. Kasaye L, Hymete A, Abdel-Maaboud IM (2010) HPTLC-densitometric method for simultaneous determination of salmeterol xinafoate and fluticasone propionate in dry powder inhalers. Saudi Pharma J 18: 153-159. 9. Nayak VG, Belapure SG, Gaitonde CD, Sule AA (1996) Determination of salmeterol in metered-dose and dry-powder inhalers by reversed-phase high performance liquid chromatography. J Pharm Biomed Anal 14: 511-513. Figure 8: Spectra of SAM 24 µg/mL , FLU 24 µg/mL and a mixture contains 12 µg/mL of each showing isosbestic point at 237.5 nm. Citation: Samir A, Salem H, Abdelkawy M (2012) Simultaneous Determination of Salmeterol Xinafoate and Fluticasone Propionate in Bulk Powder and Seritide® Diskus using High Performance Liquid Chromatographic and Spectrophotometric Method. Pharmaceut Anal Acta 3:180. doi:10.4172/2153-2435.1000180 Page 7 of 7 Volume 3 • Issue 8 • 1000180 Pharmaceut Anal Acta ISSN: 2153-2435 PAA, an open access journal Submit your next manuscript and get advantages of OMICS Group submissions Unique features: • User friendly/feasible website-translation of your paper to 50 world’s leading languages • Audio Version of published paper • Digital articles to share and explore Special features: • 200 Open Access Journals • 15,000 editorial team • 21 days rapid review process • Quality and quick editorial, review and publication processing • Indexing at PubMed (partial), Scopus, DOAJ, EBSCO, Index Copernicus and Google Scholar etc • Sharing Option: Social Networking Enabled • Authors, Reviewers and Editors rewarded with online Scientific Credits • Better discount for your subsequent articles Submit your manuscript at: www.omicsonline.org/submission 10. Murnane D, Martin GP, Marriott C (2006) Validation of a reverse-phase high performance liquid chromatographic method for concurrent assay of a weak base (salmeterol xinafoate) and a pharmacologically active steroid (fluticasone propionate). J Pharma Biomed Anal 40: 1149-1154. 11. Spencer JC, Vladimir C (2008) J Chromatog B 876: 163-169. 12. Minghua L, Zhang L, Xin L, Qiaomei L, Guonan C, et al. (2006) Talanta 81: 1655-1666. 13. Magda MA, Magda M, Henawee EL, Hisham E, Abdellatef, et al. (2005) Cairo Bull: 3. 14. Kusum M, Davinder K, Vivek T, Satish K, Love S (2011) Simultaneous Quantitative Determination of Formoterol Fumarate and Fluticasone Propionate by Validated Reversed-Phase HPLC Method in Metered dose inhaler. Der Pharmacia Sinica 6: 77-84. 15. Byrroa RM, Cesara IC, de Santana FF, Iram MM, de Souza LT, et al. (2011) A rapid and sensitive HPLC–APCI-MS/MS method determination of fluticasone in human plasma: Application for a bioequivalency study in nasal spray formulations. J Pharm Biomed analysis 61: 38-43. 16. Hiral ND, Ashlesha GM, Chaitanya B, Killambi P (2011) Validated HPTLC Method for the Determination of Two Novel steroids in Bulk and Pressurized Metered-Dose Preparations. Int J Appl Sci Eng 9: 177-185. 17. Hiral ND, Ashlesha GM (2010) Int J Pharm Health Sci 1: 68-76. 18. Lebiedzińska A, Marszałł ML, Kuta J, Szefer P (2007) Reversed-phase highperformance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B(1) (thiamine), B(6) (pyridoxamine, pyridoxal and pyridoxine) and B(12) in animal and plant foods. J Chromatogr A 1173: 71-80. 19. Nadarassan DK, Chrystyn H, Clark BJ, Assi KH (2007) Validation of highperformance liquid chromatography assay for quantification of formoterol in urine samples after inhalation using UV detection technique. J Chromatogr B Analyt Technol Biomed Life Sci 850: 31-37. 20. Luo X, Chen B, Ding L, Tang F, Yao S (2006) Anal Chim Acta 562: 185-189. 21. Salem H (2006) J J Appl Sci 828-843. 22. Mohamed A, Salem H, Maher E (2006) Thai J Pharm Sci 30: 63-81. 23. Mohamed A, Salem H, Maher E (2007) Thai J Pharm Sci 31: 1-24. 24. Salem M, El-Bardicy M, El-Tarras M, El-Zanfally E (2002) J Pharm Biomed Anal 30: 21-33. 25. Salem M, Merey H, Bayoumi A, El Zeany B (2003) Bull Fac Pharm Cairo Univ 41: 27-41. 26. Metwally F (2008) Spec Chim Acta 69: 343-349. 27. Ramadan NK, Lotfy HM (2006) Bull Fac Pharm Cairo Univ 44: 13-23. 28. Abdel-Kawy M, Amer SM, Lotfy HM, Zaazaa H (2006) Bull Fac Pharm Cairo Univ 44: 25-32.https://doi.org/10.4172/2153-2435.1000180https://t.ly/pwmEkMSA Google ScholarFive methods were developed for simultaneous determination of Salmeterol xinafoate (SAM) and Fluticasone propionate (FLU) without previous separation. In the first method (HPLC), a reversed-phase column and a mobile phase of acetonitrile: methanol (80:20 v/v) at 0.5 mLmin-1 flow rate was used to separate both drugs and UV detection at 220 nm. Linearity was obtained in concentration ranges of 50-500 µgmL-1 for Salmeterol xinafoate Fluticasone propionate. In the second method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 352 and 269.5 nm for Salmeterol xinafoate and Fluticasone propionate, respectively. The third method depends on first derivative of the ratios spectra by measurements of the amplitudes at 334 and 337.5 nm for Salmeterol xinafoate and at 225 and 231.5 nm for Fluticasone propionate. Calibration graphs were established in the range of 4-28 µgmL-1 for both Salmeterol xinafoate and Fluticasone propionate. The fourth one depend on isosbestic point at 237.5 nm, while the content of Salmeterol xinafoate was determined by measuring the absolute value of the ultraviolet curves at 343 nm, without interference from Fluticasone propionate. All the proposed methods were extensively validated. They have the advantage of being economic and time saving. All the described methods can be readily utilized for the analysis of pharmaceutical formulations. The results obtained by adopting the proposed methods were statistically analyzed and compared with those obtained by official methods.enUniversity of Salmeterol xinafoate; Fluticasone propionate; HPLC; First derivative spectrophotometry; Ratio derivative spectrophotometry; Isosbestic pointArticlehttps://doi.org/10.4172/2153-2435.1000180