Mohamed Anis, Abdul RahmanMohsen Ahmad, MaiGamal Abd EL-Nasser, Omar2019-10-102019-10-102018Copyright © 2019 MSA University. All Rights Reserved.https://t.ly/M31yAThis study aimed at Evaluation of effect of Different surface modifications of 3D printed PCL scaffolds on the immunogenicity of mesenchymal stem cells Aim: The aim of this study was the isolation and expansion of mesenchymal stem cells )MSCs (from dental pulp .and testing the effect of different surface modifications of polycaprolactone (PCL) membrane on the immunogenicity of MSCs. Methods: This In vitro study aimed at first for the isolation of MSCs from dental pulp tissue by enzyme digestion method and expanding them in culture. Second, studying of the immune phenotype of stem cells by flow cytometric analysis when exposed to surface modified scaffolds used for tissue regeneration. Data was reported as mean ± standard error. Statistical analysis was performed using Microsoft (MS) Office Excel Software. One-way ANOVA was used to asses for differences between groups, and p values were calculated by Student’s t-test. Differences were considered statistically significant if the p-value was less than 0.05 Data was reported as mean ± standard error.enOctober University for Modern Sciences and ArtsUniversity of Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابMesenchymal stem cellsRegenerative MedicineMicrobiologyOther