Moustafa A.A.Salem H.Hegazy M.Mahmoud O.A.Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini StreetCairo11562Egypt; Analytical Chemistry DepartmentFaculty of PharmacyDeraya UniversityCairo14511Egypt; Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)6th October City11787Egypt2020-01-092020-01-0920159334173https://doi.org/10.1556/1006.2015.28.4.7PubMed ID :https://t.ly/kNxZAScopusSimple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed on an Inertsil CN-3 column (250 mm � 4.6 mm, 5 ?m), using a mobile phase consisting of acetonitrile-distilled water (pH 3.5) using orthophosphoric acid in the ratio 70:30 (v/v) and flow rate of 1.5 mL min-1. Quantitation was achieved with ultraviolet (UV) detection at 220 nm. In method B, TLC analysis was carried out on an aluminum-backed sheet of silica gel 60 F254 layer using chloroform- propanol-ammonia (6:4:0.1, v/v) as the mobile. Quantification was carried out with UV detection at 245 nm. The validation of the proposed methods was applied according to the International Conference on Harmonization (ICH) guidelines. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial dosage form. � Akad�miai Kiad�, Budapest.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityCarbinoxamineEphedrinePholcodineReversed-phase high-performance liquid chromatographyThin-layer chromatography-densitometryChromatographyDrug dosageHigh performance liquid chromatographyLiquidsOrthophosphoric acidSilica gelThin layer chromatographyCarbinoxamineDensitometric methodEphedrineInternational conference on harmonizationsPholcodineReversed phase high performance liquid chromatographySimultaneous determinationsUltraviolet detectionLiquid chromatographyantitussive agentcarbinoxamineephedrinepholcodineArticlecomparative studycontrolled studydensitometerdensitometrydrug determinationflow ratelimit of detectionlimit of quantitationliquid chromatographmeasurement accuracyreproducibilityreversed phase high performance liquid chromatographythin layer chromatographyultraviolet radiationArticlehttps://doi.org/10.1556/1006.2015.28.4.7PubMed ID :