El-Hawary S.S.El-Hefnawy H.M.Osman S.M.Mostafa E.S.Mokhtar F.A.El-Raey M.A.Department of PharmacognosyFaculty of PharmacyCairo UniversityCairoEgypt; Department of PharmacognosyFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)6thOctober CityGizaEgypt; Phytochemistry and Plant Systematic DepartmentNational Research CentreDokkiCairoEgypt2020-01-092020-01-0920199757058https://doi.org/10.22159/ijap.2019v11i6.33646PubMed ID :https://t.ly/LXvXgScopusObjective: Evaluation of two Jasminum sambac L. (Ait) cultivars; Arabian Nights (JSA) and Grand Duke of Tuscany (JSG) ethanolic leaves extracts as reducing agents for the green synthesis of silver nanoparticles (AgNPs) and evaluation of their cytotoxicity against MCF-7 breast cancer and 5637 bladder cancer cell lines and chemical profiling of the two cultivars. Methods: The synthesis of silver nanoparticles (AgNPs) by the two cultivars and characterization of AgNPs by ultraviolet (UV)�visible spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the use of The high-performance liquid chromatography coupled with photodiode array-mass-mass-spectroscopy (HPLC-PDA-MS/MS) for chemical profiling of both cultivars and evaluation of total leaves extracts and corresponding nanoparticles towards MCF-7 and 5637 cell lines compared to aneuploidy immortal keratinocyte (Ha Cat) normal cells by neutral cell assay. Results: The green synthesized AgNPs (of an average size range of 8.83 and 11.24 nm for JSA and JSG, respectively) exhibited cytotoxicity against MCF-7 and 5637 cell lines. The IC50 was determined for each total extract JSA (15.29�2.16 ?g/ml) and JSG (20.28�1.20 ?g/ml) and corresponding AgNPs 17.32�2.22 ?g/ml and 6.32�1.01?g/ml for JSA and JSG, respectively. The IC50 of JSA and JSG against 5637 bladder cancer cell line were 13.76�1.11 ?g/ml and 50.69�3.75 ?g/ml, while the corresponding AgNPs showed IC50 of 5.54�0.88 ?g/ml and 27.89�2.84 ?g/ml, respectively. The HPLC-PDA-MS/MS allowed the identification of 59 compounds; 10 simple phenols, 17 flavonoids; quercetin and kaempferol glycosides, 2 lignans, and 30 secoiridoids; oleuropein, molihauside, and sambacoside. Conclusion: This study proved that JSA is an excellent source for the synthesis of AgNPs with optimum characters and enhanced activities toward MCF-7 and 5637 cell lines in correlation to identified compounds. � 2019 The Authors.EnglishUniversity for Modern Sciences and ArtsOctober University for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابAgNPsCytotoxicityGreen synthesisHPLC-PDA-MS/MSJasminum sambacetoposideJasminum sambac extractkaempferollignanoleuropeinplant extractprotocatechualdehydequercetinsecoiridoidsilver nanoparticleunclassified drugvincristine5637 cell lineArticlebladder cancerchemical fingerprintingcytotoxicitycytotoxicity assaydrug synthesisdrug therapeutic indexEgyptFourier transform infrared spectroscopyhigh performance liquid chromatographyhumanhuman cellIC50jasminekeratinocyteMCF-7 cell linespecies cultivationtransmission electron microscopyultraviolet visible spectroscopyArticlehttps://doi.org/10.22159/ijap.2019v11i6.33646PubMed ID :