Yassin G.E.Dawoud M.H.S.Wasfi R.Maher A.Fayez A.M.Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyAl Azhar UniversityCairoEgypt; Department of PharmaceuticsFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA University)GizaEgypt; Department of MicrobiologyFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA University)GizaEgypt; Department of BiochemistryFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA University)GizaEgypt; Department of PharmacologyFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA University)GizaEgypt2020-01-092020-01-0920193639045https://doi.org/10.1080/03639045.2019.1620269PubMed ID 31099273https://t.ly/vj06YScopusPlatelet-rich plasma (PRP) accelerates wound healing, as it is an excellent source of growth factors. PRP was separated from whole human blood by centrifugation. PRP powder and wafers were prepared by lyophilization, with the wafers prepared using sodium carboxymethylcellulose (Na CMC). The PRP wafers showed porous structures, as indicated by scanning electron microscopy (SEM) images, and the ability of the wafer to absorb exudates and thus promote wound healing was tested with the hydration capacity test. The platelet count was tested and indicated that the presence of PRP in the wafers had no effect on the platelet count. An antimicrobial activity test was carried out, showing that PRP had antibacterial activity against Gram-negative bacteria. Compared with lyophilized PRP powder and PRP-free wafers, PRP wafers showed the highest percent of wound size reduction on induced wounds in rats. Histopathological examination of rat skin showed that the PRP wafers achieved the shortest healing time, followed by the lyophilized PRP powder and finally the PRP-free wafers. The present study revealed that PRP can be formulated as a wafer, which is a promising pharmaceutical delivery system that can be used for enhanced wound-healing activity and improved the ease of application compared to lyophilized PRP powder. 2019, 2019 Informa UK Limited, trading as Taylor & Francis Group.Englishhistopathological studylyophilization (freeze-drying)Platelet-rich plasma (PRP)waferwound healingcarboxymethylcellulosewound healing promoting agentantiinfective agentAcinetobacter baumanniianimal cellanimal experimentanimal tissueantibacterial activityArticlebacterial growthcontrolled studydrug delivery systemepithelizationfreeze dryinghistopathologyhumanhuman cellhydrationin vitro studyin vivo studymalenonhumanphysical appearanceplatelet countpowderratscanning electron microscopythrombocyte rich plasmawaferwater losswound closurewound healingzone of inhibitionadministration and dosageanimalchemistrydrug effectfreeze dryingGram negative bacteriumGram negative infectionmicrobiologypowderproceduresskinthrombocyte rich plasmaWistar ratwound healingAnimalsAnti-Bacterial AgentsCarboxymethylcellulose SodiumFreeze DryingGram-Negative BacteriaGram-Negative Bacterial InfectionsMalePlatelet-Rich PlasmaPowdersRatsRats, WistarSkinWound HealingArticlehttps://doi.org/10.1080/03639045.2019.1620269PubMed ID 31099273