Riad S.M.El-Rahman M.K.A.Fawaz E.M.Shehata M.A.Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini StreetCairo11562Egypt; Analytical Chemistry DepartmentFaculty of PharmacyOctober for Modern Sciences and Arts University6th of October CityEgypt2020-01-092020-01-09201513861425https://doi.org/10.1016/j.saa.2015.03.030PubMed ID 25791882https://t.ly/2dPx5ScopusAbstract Three sensitive, selective, and precise stability indicating spectrophotometric methods for the determination of the X-ray contrast agent, diatrizoate sodium (DTA) in the presence of its acidic degradation product (highly cytotoxic 3,5-diamino metabolite) and in pharmaceutical formulation, were developed and validated. The first method is ratio difference, the second one is the bivariate method, and the third one is the dual wavelength method. The calibration curves for the three proposed methods are linear over a concentration range of 2-24 ?g/mL. The selectivity of the proposed methods was tested using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of DTA in pharmaceutical dosage forms without interference from other dosage form additives. The results were statistically compared with the official US pharmacopeial method. No significant difference for either accuracy or precision was observed. 2015 Elsevier B.V. All rights reserved.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityBivariateDual wavelengthRatio differenceSodium diatrizoateStability indicating methodsDegradationPhotodegradationSodiumSpectrophotometersBivariateDiatrizoateDual wavelengthRatio differencesStability indicating methodSpectrophotometrydiatrizoatemeglumine diatrizoatecell deathchemistrycomparative studydrug effectslightproceduresreproducibilityspectrophotometrystandardCell DeathDiatrizoateDiatrizoate MeglumineLightReference StandardsReproducibility of ResultsSpectrophotometryArticlehttps://doi.org/10.1016/j.saa.2015.03.030PubMed ID 25791882