Shahat E.A.Bakr R.O.Eldahshan O.A.Ayoub N.A.Pharmacognosy DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)GizaEgypt; Pharmacognosy DepartmentFaculty of PharmacyAin Shams UniversityCairoEgypt; Pharmacology DepartmentFaculty of MedicineUmm Al-Qura UniversityMakkah24382Saudi Arabia2020-01-092020-01-09201716121872https://doi.org/10.1002/cbdv.201600156PubMed ID 28094467https://t.ly/1VVwlScopusThe composition of the essential oil isolated from leaves and flowers of Pulicaria incisa sub. candolleana E. Gamal-Eldin, growing in Egypt, was analysed by GC and GC-MS. Forty-nine and 68 compounds were identified from the oils of the leaves and flowers accounting for 86.69 and 84.29%, respectively of the total detected constituents. Both leaves and flowers oils were characterized by the high content of carvotanacetone with 66.01, 50.87 and chrysanthenone 13.26, 24.3%, respectively. The cytotoxic activity of both essential oils was evaluated against hepatocellular carcinoma cell line HEPG-2, using MTT assay and vinblastine as a reference drug. Leaf oil showed higher activity with IC50 11.4�?g/ml compared with 37.4�?g/ml for flower oil. The antimicrobial activity of both oils was evaluated using agar well diffusion method towards two representatives for each of Gram positive and Gram negative bacteria as well as four representatives for fungi. The minimum inhibitory concentration of both essential oils against bacterial and fungal strains was obtained in the range of 0.49���15.63�?g/ml. � 2017 Wiley-VHCA AG, Zurich, SwitzerlandEnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابAntimicrobial activitiesCytotoxic activitiesEssential oilGC-MS AnalysisPulicaria incisa sub. candolleana1 octen 3 ol1,2,4 trimethylbenzenealpha thujeneamphotericin Bampicillinanetholeantibiotic agentantifungal agentbeta pinenebourbonenecamphenecarveolcineolecopaenecytotoxic agentessential oilgentamicinhexanalhexanolhexobarbitaljasmonelauric acidpalmitic acid isopropyl esterpinenesabinenesesquiterpeneterpinen 4 olterpineneunindexed drugvinblastine sulfateantifungal agentantiinfective agentantineoplastic agentborane derivativechrysanthenoneessential oilantibacterial activityantifungal activityArticleAspergillus fumigatusBacilliBacillus subtilisbacterial strainCandida albicanschemical compositioncomparative studyconcentration responsecontrolled studycytotoxicityEscherichia coliflowerfungal strainGeotricum candidumHep-G2 cell lineIC50minimum inhibitory concentrationMTT assaynonhumanplant leafPseudomonas aeruginosaPulicariaPulicaria incisa sub. candolleanaPulicaria inuloidesPulicaria jaubertiiPulicaria stephanocarpaStreptococcus pneumoniaeSyncephalastrum racemosumchemistrydrug effectsEgyptflowerGram negative bacteriumGram positive bacteriumhumanisolation and purificationmass fragmentographymicrobial sensitivity testplant leafPulicariaAnti-Bacterial AgentsAntifungal AgentsAntineoplastic AgentsBoranesEgyptFlowersGas Chromatography-Mass SpectrometryGram-Negative BacteriaGram-Positive BacteriaHep G2 CellsHumansMicrobial Sensitivity TestsOils, VolatilePlant LeavesPulicariaArticlehttps://doi.org/10.1002/cbdv.201600156PubMed ID 28094467