Al-Gendy A.A.Nematallah K.A.Zaghloul S.S.Ayoub N.A.Faculty of PharmacyOctober University for Modern Sciences and Arts (MSA)GizaEgypt; Faculty of PharmacyZagazig UniversityZagazigEgypt; Faculty of PharmacyBritish University in EgyptCairoEgypt; Faculty of PharmacyAin-Shams UniversityCairoEgypt; Faculty of MedicineUmm Al-Qura UniversityMakkahSaudi Arabia2020-01-092020-01-09201613880209https://doi.org/10.1080/13880209.2016.1223146PubMed ID 27597660https://t.ly/ndL5rScopusContext: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0�160 ?g/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01�100 ?g/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC = 64 and 82 ?g/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 ?g/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. � 2016 Informa UK Limited, trading as Taylor & Francis Group.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابGC-MSglucoerucinglucoiberinGlucoiberverinLC-MSsulforhodamine Bantiinfective agentdoxorubicinglucosinolateprotein hydrolysatesulforhodamine Bvolatile agentcytotoxinglucosinolateplant extractantimicrobial activityArticleBrassicaceaebreast cancerbreast cancer cell lineCandida albicanscolorectal cancercontrolled studycytotoxicitydisk diffusiongas chromatographyhumanhuman cellhydrolysisIC50in vitro studyliquid chromatographyliver cancerLobularia libycalung cancermass spectrometryminimum inhibitory concentrationplant leafplant rootplant seedPseudomonas aeruginosaA-549 cell lineBrassicaceaecell survivaldose responsedrug effectHCT 116 cell lineisolation and purificationMCF-7 cell linemicrobial sensitivity testphysiologyvolatilizationA549 CellsAnti-Infective AgentsBrassicaceaeCell SurvivalCytotoxinsDose-Response Relationship, DrugGlucosinolatesHCT116 CellsHumansMCF-7 CellsMicrobial Sensitivity TestsPlant ExtractsPlant LeavesPlant RootsSeedsVolatilizationArticlehttps://doi.org/10.1080/13880209.2016.1223146PubMed ID 27597660