Gizawy S.M.Bebawy L.I.Abdelmageed O.H.Omar M.A.Deryea S.M.Abdel-Megied A.M.Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyAssuit UniversityAssuitEgypt; National Organization for Drug Control and ResearchBen-El-SaraiatDokkiGizzaEgypt; Pharmaceutical Chemistry DepartmentFaculty of PharmacyKing Abdulaziz UniversityJeddahSaudi Arabia; Analytical Chemistry DepartmentFaculty of PharmacyMinia UniversityMiniaEgypt; October University for Modern Sciences and Arts (MSA)Pharmaceutical Analytical ChemistryWahat RoadGizah 61111Egypt2020-01-092020-01-09201310826076https://doi.org/10.1080/10826076.2012.686141PubMed ID :https://t.ly/j62bPScopusThree simple, sensitive, and specific methods were developed for simultaneous determination of amlodipine besylate (AML) and Perindopril Erbumine (PER) without previous separation. The first method was dependent on the first derivative of the ratio spectra by measuring the amplitudes at 348 nm for amlodipine using 50 g mL-1 of perindopril as a divisor and at 227 nm for perindopril using 30 g mL-1 of amlodipine as a divisor. The second method was based on ion-pair RP-HPLC. Satisfactory resolution was achieved using RP-C18 chromatographic column Zorbax Extend column and a mobile phase consists of potassium dihydrogen phosphate buffer (0.05 M, pH 3.0 � 0.02 adjusted by orthophosphoric acid): acetonitrile 30:70 v/v at a flow rate 1 mL/min using 0.002 M sodium heptanesulfonate in the aqueous phase. UV detection was performed at 215 nm. The third method was based on TLC; the separation was carried out on Fluka TLC aluminum sheets silica gel 60 F254, using n-butanol:water:glacial acetic acid (4:5:1, v/v/v) as the mobile phase. The validation of the proposed methods was applied according to ICH guidelines and LOD and LOQ were calculated. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial tablets. � 2013 Copyright Taylor and Francis Group, LLC.Englishamlodipine besylateCOVERAM tabletsderivative ratio spectrophotometryperindopril erbumineRP-HPLCTLS-DensitometryAmlodipine besylateCOVERAM tabletsDerivative ratiosperindopril erbumineRP-HPLCTLS-DensitometryHigh performance liquid chromatographySeparationSilica gelSpectrophotometryChromatographyacetic acidacetonitrileamlodipine besylatebutanolperindopril tert butylaminephosphoric acidpotassium dihydrogen phosphatesodium heptanesulfonatesulfonic acid derivativeunclassified drugwateramplitude modulationarticledensitometrydrug determinationflow ratehigh performance liquid chromatographyion pair chromatographypowderreal time polymerase chain reactionsatisfactionspectrophotometrytablet formulationultraviolet spectrophotometryvalidation processArticlehttps://doi.org/10.1080/10826076.2012.686141PubMed ID :