El-Mosalamy H.Salman T.M.Ashmawey A.M.Osama N.Biochemistry DepartmentFaculty of PharmacyAin Shams UniversityCairoEgypt; Biochemistry DepartmentFaculty of PharmacyAl-Azhar UniversityCairoEgypt; Cancer Biology DepartmentNational Cancer InstituteCairo UniversityCairoEgypt; Biochemistry DepartmentFaculty of PharmacyModern Sciences and Arts UniversityCairoEgypt2020-01-252020-01-25201217509378https://doi.org/10.1186/1750-9378-7-19PubMed ID :https://t.ly/BX3KLScopusBackground: Bladder cancer is a common malignancy in Egypt. A history of urinary tract infection can be considered as a risk factor for bladder cancer. Escherichia coli (E. coli) infection is responsible for 70% of urinary tract infection. This study aimed to evaluate the role of chronic E. coli infection during bladder carcinogenesis. In order to achieve this aim, we investigated the histopathological changes in bladder tissue and measured the level of nuclear factor kappa p65 (NF-Bp65), Bcl-2 and interleukin 6 (IL-6) in four groups each consisting of 25 male albino rats except of control group consisting of 20 rats. The first group was normal control group, the second group was infected with E. coli, the third group was administered nitrosamine precursor, and the forth group was infected with E. coli and administered nitrosamine precursor. Results: The histopathological examination revealed that E. coli infected group was able alone to produce some histopathological changes in bladder tissue and that nitrosamine precursor plus E. coli group showed highest incidences of urinary bladder lesions than the nitrosamine precursor group. NF-Bp65, Bcl-2 and IL-6 levels were significantly higher in nitrosamine precursor plus E. coli group than the other groups. Conclusion: These findings suggested that urinary bladder infection by E. coli may play a major additive and synergistic role during bladder carcinogenesis. � 2012 El-Mosalamy et al.; licensee BioMed Central.EnglishBcl-2Bladder carcinogenesisE. coliIL-6NF-Bp65interleukin 6nitrosamineprotein bcl 2transcription factor RelAanimal experimentanimal modelanimal tissuearticlebladder cancercontrolled studyEscherichia coli infectionexperimental studyhistopathologymalenonhumanpathophysiologypriority journalraturinary tract infectionArticlehttps://doi.org/10.1186/1750-9378-7-19PubMed ID :