El A Helal, ThanaaEl Abdel Wahab, Hoda ESaber, Sally MAbdelaaty, Waleed HEltabbakh, Mohamed MAref, Ahmed MDawood, Mohamed H2022-05-252022-05-252022-03https://doi.org/10.4314/ahs.v22i1.70http://repository.msa.edu.eg/xmlui/handle/123456789/4954SJR : Scientific Journal Rankings - Scimagohttps://bit.ly/3GreMtzBackground/Aim: Ulcerative Colitis (UC) is an inflammatory bowel disease which is common in many areas of the world including Egypt. A lot of controversy regarding the pathogenesis of UC exist. The current study is an attempt to detect some pathogenic bacteria in UC patients. Materials and methods: Endoscopic colonic biopsies obtained from 40 patients with ulcerative colitis and 20 controls were analyzed by means of real-time PCR technique for the presence of Clostridium difficile, Helicobacter Pylori (H. pylori) and pathogenic Escherichia Coli (E. coli) which are positive for KPC and/or OXA-48. Results: All patients and control samples were negative for Clostridium difficile. Three of the 40 patient samples (7.5%) and none of the 20 controls were positive for H. pylori with no significant difference between the two groups. KPC-positive E. coli were detected in 11 of the 40 patients (27.5%) and in none of the controls with a significant difference between the two groups (P=0.01). All patients and control samples were negative for OXA-48 positive E. coli. Conclusion: Although this study does not support the claim that Clostridium difficile and/or H. pylori have a role in UC, it greatly suggests that pathogenic E. coli may be involved in one way or another in the course of UC.en-USUlcerative colitisColonic biopsiesClostridium difficileH. pyloriE. coliMolecular detection of pathogenic bacteria in the colonic biopsies from patients with Ulcerative ColitisArticlehttps://doi.org/10.4314/ahs.v22i1.70