Faculty Of Biotechnology Graduation Project 2019 - 2020

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Browsing Faculty Of Biotechnology Graduation Project 2019 - 2020 by Subject "BioTechnology"

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    Determination of malachite green, crystal violet, and their metabolites in canned fish tissues HPLC-MS/MS and its contribution in dietary intake
    (October University for Modern Sciences and Arts, 2020) Ayman Hussein, Afaf
    Multiresidues of illegal dyes are found in canned products. Illegal dyes are used as veterinary drugs. This study evaluates malachite green (MG), crystal violet (CV) and naturally reduced form leucomalachite green (LMG) and leucocrystal violet (LCV). These dyes are prohibited in processed fishes, due to their apprehensions on human and fish health for example, toxicity, carcinogens and teratogens. However, they still used extensively worldwide, because they act as antifungal and antibacterial agents. Illegal dyes are stable even after long time of exposure to heat. This evaluation was done on 30 different samples of canned fish tissues from various companies. Liquid chromatography mass spectrometry (LC-MS/MS) was the techniques used to determine MG, LMG, CV and LCV by C 18 column and Quick, Easy, Cheap, Effective, Rugged and Safe method (QuEChERS) using acetonitrile in extraction. The consternations of total MG, CV and LCV were 0.92 g/Kg, 0.1 g/Kg and LCV was not detected, where below detected limit respectively. MG, LMG, CV and LCV residues were not exceeding the European Union minimum required performance limited (MRPL) which is 2 g/Kg. Therefore, the results were indicating that Egyptian local markets control the usage of MG, CV, LMG and LCV. In addition, it provides reliable data about the actual level of the contaminant limit. From our study, accomplish that the samples collected from various Egyptian markets indicate the companies use accurate amount of MG, CV, LMG and LCV are not exceeding the permitted European Union limits. Key words: malachite green, crystal violet, mass spectrometry.
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    Evaluation of antibacterial activity of chitosan-coated anisotropic silver Nanoparticles on different bacterial strains
    (October University for Modern Sciences and Arts, 2020) Saeed Abdelmonem Elqiey, Ahmed
    The present study explores the antibacterial activity of Chitosan NPs when it is coated with different concentrations of silver NPs. CS-AgNPs materials were synthesized by a simple chemical method. The synthesized CS–Ag Nanoparticles contains two different ratio of silver to be able to demonstrate the effect of different concentrations of silver on the antibacterial activity of the synthesized product. Silver Nanoparticles were synthesized by chemical reduction method as well. The CS–Ag Nanoparticles was characterized using Fourier transform infrared spectroscopy (FTIR) that was used to study the conformational changes in the functional groups of the polymers present in CS-AgNPs, in addition to Transmission electron microscope (TEM) for determining The morphology and size, also atomic force microscopy (AFM) to provide topographical chart for the synthesized product, as well as X-ray diffraction (XRD) to investigate The crystalline and phase structure of CS-AgNPs. FTIR spectroscopy has confirmed the presence of both silver and chitosan Nanoparticles as it was also observed. TEM results have revealed that CS-AgNPs has a well capped multi structures and well distribution of silver on the nanoparticle surface. AFM results have indicated that silver NPs is well dispersed and loaded into the chitosan sheet. XRD pattern of CS-AgNPs has shown an amorphous shape for the product that was conducted from the broadening of peaks. The results have clearly indicated the interaction between chitosan and metallic surface of silver NPs which has led to effective stabilization of silver NPs on the chitosan polymer. CS-AgNPs antibacterial activity was assessed by measuring the inhibition zone when it was tested against Bacillus subtitles, Bacillus lichniforms, E. coli, and Enterobacter ludwigii, and has showed highly potent antibacterial activity.