Browsing by Author "Zahran F."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Association of serum level of Vitamin D and VDR polymorphism Fok1 with the risk or survival of pancreatic cancer in Egyptian population
    (Wolters Kluwer Medknow Publications, 2019) Mohamed A.; Aref A.; Talima S.; A Elshimy R.; Gerges S.; Meghed M.; Zahran F.; El-Adawy E.; Abd-Elsalam S.; Department of Biochemistry and Molecular Biology; National Hepatology and Tropical Medicine Research institute; Nasr City; Cairo; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts (MSA); Giza City; Giza; Egypt; Department of Clinical Oncology; Kasr Al-Ainy Center of Clinical Oncology and Nuclear Medicine (NEMROCK); Kasr Al-Ainy School of Medicine; Cairo University; Giza City; Giza; Egypt; Department of Clinical Pathology; National Cancer Institute; Cairo University; Cairo Governorate; Mansoura City; Egypt; Department of Surgery; National Hepatology and Tropical Medicine Research institute; Nasr City; Cairo; Egypt; Department of Internal Medicine; Faculty of Medicine (Girls); Al-Azhar University; Nasr City; Cairo; Egypt; Department of Internal Medicine and Endocrinology; Faculty of Medicine; Mansoura University; Elgomhouria St; Mansoura City; Egypt; Department of Tropical Medicine; Faculty of Medicine Tanta University; l-Gaish; Tanta Qism 2; Tanta; Gharbia Governorate; Egypt
    BACKGROUND AND AIMS: Pancreatic cancer (PC) is the fourth most common cause of death from cancer in Egypt. Few studies have been conducted to assess the relationship between vitamin D serum level and vitamin D receptor (VDR) polymorphisms with the survival of PC patients. This is the first study in Egypt to investigate the association of the status of vitamin D serum level and genotypic distribution of single nucleotide polymorphisms (SNP) Fok1 with the risk of developing PC and whether they could detect survival or not. PATIENTS AND METHODS: The study included a total of 47 PC cases that were histopathologically proven to have PC, and 37 controls that were attending at the same time for investigation but proved that they were all PC free. Pre-diagnostic concentrations of vitamin D and VDR polymorphism Fok1 were assessed from all participants in the study. RESULTS: There was a 1.5-fold increase in the serum level of vitamin D in PC patients when compared to non-PC subjects. Regarding VDR Fok1, polymorphism distribution in PC was CC (Wild Type) 26 (55.3%), CT 16 (34%), and TT 5 patients (10.7%). For the control group, CC was found in 24 (64.8%), CT in 12 (32.4%), and TT genotype was found only in one individual 1 (2.8%) with no statistically significant difference between the two studied groups (P 0.72). CONCLUSION: Low serum vitamin D or VDR-SNP is not a risk factor for PC in Egyptian patients. Recommendations to increase vitamin D concentrations in healthy persons for the prevention of cancer and improving overall survival should be carefully considered. 2019 Indian Journal of Cancer | Published by Wolters Kluwer -Medknow.
  • Thumbnail Image
    Item
    Salivary microRNAs in oral cancer
    (Blackwell Publishing Ltd, 2015) Zahran F.; Ghalwash D.; Shaker O.; Al-Johani K.; Scully C.; Division of Oral Medicine; Oral Diagnostic Sciences Department; Faculty of Dentistry; King Abdulaziz University; Jeddah; Saudi Arabia; Department of Oral Medicine and Periodontology; Faculty of Oral and Dental Medicine; Cairo University; Cairo; Egypt; Department of Oral Medicine and Periodontology; Faculty of Dentistry; October University for Modern Sciences and Arts; 6th October City; Egypt; Department of Medical Biochemistry; Faculty of Medicine; Cairo University; Cairo; Egypt; UCL; London; United Kingdom
    Objective: This study investigated the use of three salivary microRNAs (miRNA-21, miRNA-184, and miRNA-145) as possible markers for malignant transformation in oral mucosal lesions. Materials and methods: Salivary whole unstimulated samples were collected from a study group of 100 subjects, consisting of 20 clinically healthy controls, 40 patients with oral potentially malignant disorders (PMDs) [20 with dysplastic lesions and 20 without dysplasia], 20 with biopsy-confirmed oral squamous cell carcinoma (OSCC), and 20 with recurrent aphthous stomatitis (RAS) as disease controls. Total RNA was isolated and purified from saliva samples using the microRNA Isolation Kit (Qiagen, UL). miRNA expression analysis was performed using qRT-PCR (Applied Biosystems). Results: There was a highly significant increase in salivary miRNA-21 and miRNA-184 in OSCC and PMD (with and without dysplasia) when compared to healthy and disease controls (P�<�0.001). Conversely, miRNA-145 levels showed a highly significant decrease in OSCC and PMD overall (P�<�0.001). RAS cases showed no significant difference from normal controls in any measured miRNA (P�>�0.05). The only microRNA to discriminate between OSCC and PMD with dysplasia was miRNA-184. When receiver operating characteristic curves were designed for the three miRNAs, cutoff points delineating the occurrence of malignant change were a fourfold increase in miRNA-21 with specificity 65% and sensitivity 65%, a 0.6 decrease in miRNA-145, with specificity 70% and sensitivity 60%, and a threefold increase of miRNA-184, with specificity 75% and sensitivity 80%. Calculating the area under the curve revealed that miRNA-184 was the only one among the studied miRNAs that provided good diagnostic value. Conclusion: Salivary determination of the miRNAs tested might furnish a noninvasive, rapid adjunctive aid for revealing malignant transformation in oral mucosal lesions, particularly miRNA-184. � 2015 John Wiley & Sons A/S.