Browsing by Author "Zafer M.M."

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Antimicrobial resistance pattern and their beta-lactamase encoding genes among Pseudomonas aeruginosa strains isolated from cancer patients
(2014) Zafer M.M.; Al-Agamy M.H.; El-Mahallawy H.A.; Amin M.A.; Ashour M.S.E.-D.; Department of Microbiology and Immunology; Faculty of Pharmacy; Ahram Canadian University; Cairo; Egypt; Department of Pharmaceutics and Microbiology; College of Pharmacy; King Saud University; P.O. Box 2457; Riyadh 11451; Saudi Arabia; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Clinical Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Science and Arts University; 6th October City; Giza; Egypt
This study was designed to investigate the prevalence of metallo-?-lactamases (MBL) and extended-spectrum ?-lactamases (ESBL) in P. aeruginosa isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122 P. aeruginosa isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for P. aeruginosa to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122 P. aeruginosa, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of bla VIM2, bla OXA10-, bla VEB1, bla NDM-, and bla IMP1-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion, bla VIM2- and bla OXA10-like genes were the most prevalent genes in P. aeruginosa in Egypt. To our knowledge, this is the first report of bla VIM2, bla IMP1, bla NDM, and bla OXA10 in P. aeruginosa in Egypt. � 2014 Mai M. Zafer et al.
First report of NDM-1-producing Pseudomonas aeruginosa in Egypt
(Elsevier, 2014) Zafer M.M.; Amin M.; ElMahallawy H.; Ashour M.S.E.D.; AlAgamy M.; Department of Microbiology and Immunology; Faculty of Pharmacy; Ahram Canadian University; 4th Industrial Zone; Banks Complex; 6th of October; Giza; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; El Aini; Al Sayedah Zeinab; Cairo; Egypt; Department of Clinical Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; MSA University; 6th of October; Giza; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Microbiology; College of Pharmacy; King Saud University; Riyadh; Saudi Arabia
This work reports the occurrence of New Delhi metallo-beta-lactamase 1 (NDM-1) in metallo-beta-lactamase-producing Pseudomonas aeruginosa in Egypt for the first time, and the presence of more than one blaMBL gene in carbapenem-resistant P. aeruginosa. � 2014 The Authors.
Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans
(Blackwell Publishing Inc., 2018) Wasfi R.; Abd El-Rahman O.A.; Zafer M.M.; Ashour H.M.; Department of Microbiology and Immunology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University (Girls); Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Ahram Canadian University (ACU); Giza; Egypt; Department of Biological Sciences; College of Arts and Sciences; University of South Florida St. Petersburg; St. Petersburg; FL; United States; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; Cairo; Egypt
Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lactobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lactobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR). Growth changes (OD600) in the presence of pH-neutralized, catalase-treated or trypsin-treated Lactobacillus sp. supernatants were assessed to identify roles of organic acids, peroxides and bacteriocin. Susceptibility testing indicated antibacterial (pH-dependent) and antibiofilm activities of Lactobacillus sp. against Streptococcus mutans. Scanning electron microscopy revealed reduction in microcolony formation and exopolysaccharide structural changes. Of the oral normal flora, L.salivarius exhibited the highest antibiofilm and peroxide-dependent antimicrobial activities. All biofilm-forming cells treated with Lactobacillus sp. supernatants showed reduced expression of genes involved in exopolysaccharide production, acid tolerance and quorum sensing. Thus, Lactobacillus sp. can inhibit tooth decay by limiting growth and virulence properties of Streptococcus mutans. � 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.