Browsing by Author "Yassin G.E."

Now showing 1 - 5 of 5

Carbamazepine loaded vesicular structures for enhanced brain targeting via intranasal route: Optimization, in vitro evaluation, and in vivo study
(Innovare Academics Sciences Pvt. Ltd, 2019) Yassin G.E.; Amer R.I.; Fayez A.M.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al Azhar University; Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Department of Pharmacology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt
Objective: Carbamazepine (CBZ) is used as a first line in the treatment of grand mal and partial seizures, but it suffers from many side effects on different systems of the body. The objective of the present study was optimization of CBZ vesicular structures using 23 multifactorial design for the most efficient targeting of CBZ to the brain via the intranasal route. Methods: The concentration of CBZ (10 and 20%), type of vesicles (niosomes and spanlastics) and speed of rotation (200 and 300 rpm) were considered as the independent variables XA, XB and XC respectively, while the dependent variables were particle size PS (Y1), polydispersity index PDI (Y2), zeta potential ZP (Y3) and entrapment efficiency EE (Y4). The study of the effect of different formulation variables was carried out using Design-Expert �� software. CBZ-loaded spanlastics and noisome were prepared by the ethanol injection method and thin film hydration method, respectively. The optimized formulation was subjected to viscosity measurement, in vitro drug release and physical stability studies. In vivo evaluations in rats for the optimized formulation in comparison to oral CBZ suspension was carried out using behavioral assessment by elevated plus maze test, determination of endothelial nitric oxide synthase (e-NOS), reduced glutathione (GSH) and ELISA estimation of TNF��. Results: The selected optimized formulation (F0) containing 20% CBZ and spanlastic vesicular structure showed PS, PDI, ZP, and the EE % of 350.09 nm, 0.830, 16.124mV and 82.777%, respectively. In vitro release study of F0 demonstrated the ability of the F0 to increase drug release in the range time from 10-60 min (p<0.05) when compared with CBZ suspension. The viscosity of F0 was nearly uniform (65 cps). The photomicrograph taken by the transmission electron microscopy (TEM) reveals the spherical shape of F0. Good physical stability for six months of storage at 25�a C was found for F0. The optimized spanlastic formulation F0 showed a decrease in latency time in behavior assessment test using elevated plus Maze test, a decrease in serum eNOS and TNF-�� and increase in GSH when compared with the oral CBZ suspension, in addition to the histopathological study that revealed the more CBZ uptake by the brain. Conclusion: The optimized spanlastic formulation F0 achieved better results when compared with the oral CBZ suspension for targeting the CBZ spanlastics vesicular structure to the brain via the nasal route. � 2019 The Authors.
Comparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, in vitro and in vivo studies
(Taylor and Francis Ltd., 2019) Yassin G.E.; Dawoud M.H.S.; Wasfi R.; Maher A.; Fayez A.M.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al Azhar University; Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Microbiology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Pharmacology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt
Platelet-rich plasma (PRP) accelerates wound healing, as it is an excellent source of growth factors. PRP was separated from whole human blood by centrifugation. PRP powder and wafers were prepared by lyophilization, with the wafers prepared using sodium carboxymethylcellulose (Na CMC). The PRP wafers showed porous structures, as indicated by scanning electron microscopy (SEM) images, and the ability of the wafer to absorb exudates and thus promote wound healing was tested with the hydration capacity test. The platelet count was tested and indicated that the presence of PRP in the wafers had no effect on the platelet count. An antimicrobial activity test was carried out, showing that PRP had antibacterial activity against Gram-negative bacteria. Compared with lyophilized PRP powder and PRP-free wafers, PRP wafers showed the highest percent of wound size reduction on induced wounds in rats. Histopathological examination of rat skin showed that the PRP wafers achieved the shortest healing time, followed by the lyophilized PRP powder and finally the PRP-free wafers. The present study revealed that PRP can be formulated as a wafer, which is a promising pharmaceutical delivery system that can be used for enhanced wound-healing activity and improved the ease of application compared to lyophilized PRP powder. 2019, 2019 Informa UK Limited, trading as Taylor & Francis Group.
Design and evaluation of fast dissolving oro-dispersible films of metoclopramide hydrochloride using 32 multifactorial designs
(Innovare Academics Sciences Pvt. Ltd, 2016) Yassin G.E.; Abass H.A.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Science and Arts (MSA) University; Egypt
Objective: The objective of the present work was to develop and optimize fast dissolving orodispersible films containing metoclopramide hydrochloride using 32 multifactorial designs. Methods: The films were prepared by solvent casting method using hydroxypropyl methyl cellulose E5 (HPMC E5) and sodium starch glycolate (SSG) as two independent variables in three levels in concentration 2.5, 3, 3.5% w/wand 1, 1.5, and 2 % respectively. The percent of in vitro drug release (Y1) and the disintegration time (Y2) were chosen and studied as dependent responses. The prepared films were also evaluated for their weight uniformity, thickness, surface pH, drug content, in vitro disintegration time, in vitro drug release, film stability and mechanical properties as folding endurance. Results: All the films were transparent. The films weight (mg) was ranging from 63. 0.78 to 86. 0.82 while the film thickness (mm) and the folding endurance range from 0.22. 0.53, 50.. 0.58 to 0.32. 0.35 and 90. 0.84 respectively. The drug content (mg %) was studied, and it ranges from 98.24. 1.08 to 99.07. 1.02. It was found that the relative standard deviation (% RSD) met the criteria of USP specification for drug content (>6%). In vitro disintegration time was tested; all films satisfied the requirement of disintegration time for fast dissolving dosage form (<1 min), it ranged from 2.24. 1.75 to 3.18. 1.87 sec. The stability studies revealed no significant differences before and after storage for the all formulations. Conclusion: An optimized metoclopramide HCl film was achieved that could be a benefit to a patient suffering from emesis, in which hydroxypropyl methylcellulose (E5) was used as a film forming polymer in its high level (3.5%) in addition to sodium starch glycolate in its high level (2%). 2016 The Authors.
Insulin Mucoadhesive Liposomal Gel for Wound Healing: a Formulation with Sustained Release and Extended Stability Using Quality by Design Approach
(Springer New York LLC, 2019) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts; (MSA University); Giza; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
The present study deals with the formulation of topical insulin for wound healing with extended stability and sustained release, by applying quality by design concepts. Insulin has been promoted as a promising therapeutic wound healing agent. Topical formulation of insulin faced major problems, as it cannot be delivered safely to the wound with a controlled rate. Formulation of insulin-loaded vesicles in optimized bio-adhesive hydrogels has been explored to ensure a safe delivery of insulin to wounds in a controlled manner. Quality by design (QbD) was applied to study the effect of several critical process parameters on the critical quality attributes. Ishikawa diagram was used to identify the highest risk factors, which were screened by a fractional factorial design and augmented by Box�Behnken design. The optimized formula was incorporated into a mucoadhesive gel, which was further subjected to stability and clinical studies. An optimized formula was obtained with a particle size of 257.751�nm, zeta potential ? 20.548�mv, 87.379% entrapment efficiency, and a release rate of 91.521�?g/cm2/h. The results showed that liposomal insulin remained stable for 6�months in aqueous dispersion state at 4�C. Moreover, the release was sustained up to 24�h. The clinical study showed an improvement in the wound healing rate, 16 times, as the control group, with magnificent reduction in the erythema of the ulcer and no signs of hypoglycemia. Insulin-loaded liposomal chitosan gel showed a promising drug delivery system with high stability and sustained release. � 2019, American Association of Pharmaceutical Scientists.
Response surface optimization and in-vitro evaluation of sustained release topical insulin liposomal spray for wound healing
(Open Science Publishers LLP Inc., 2018) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
Chronic wounds are considered a major health care concern, that represents a life-threatening problem worldwide. Insulin has proven its great efficiency as a wound healing agent, especially with diabetic ulcers. However; insulin suffers degradation at the application site due to proteases, moreover; when wounds are painful the patient fails to apply any remedy frequently. In the present study, insulin has been formulated as a spray in liposomes, which protects it at the wound area and sustains its release and thus reducing the application frequency, furthermore; the spray reducing the direct contact of the applicator with the skin, thus, reducing the probability of infection. The full-factorial design has been applied in the preparation optimization of liposomes, where the effects of the cholesterol, method of preparation and sonication have been tested on the particle size and the entrapment efficiency. The present study shows how the thin film hydration method in the absence of cholesterol and sonication were the best conditions for insulin. liposomal formulation, that satisfies the target of the study. Liposomes showed a sustained release of insulin up to 24 hours and were successfully formulated into a spray dosage form. In conclusion, topical insulin liposomal spray offers a protective method from insulin degradation with an expected increase in the patient compliance. � 2018 Marwa H. S. Dawoud et al.