Browsing by Author "Tester M."

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    Improved salinity tolerance of rice through cell type-specific expression of ATHKT1;1
    (2010) Plett D.; Safwat G.; Gilliham M.; Miller I.S.; Roy S.; Shirley N.; Jacobs A.; Johnson A.; Tester M.; Australian Centre for Plant Functional Genomics; University of Adelaide; Glen Osmond; SA; Australia; School of Agriculture. Food and Wine; Waite Research Institute; University of Adelaide; Glen Osmond; South Australia; Australia; Horticulture Research Institute; Agriculture Research Centre; Cairo; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; Cairo; Egypt; Department of Agriculture and Ecology; Faculty of Life Sciences; University of Copenhagen; Frederiksberg; Denmark; School of Botany; University of Melbourne; Melbourne; VIC; Australia
    Previously, cell type-specific expression of AtHKT1;1, a sodium transporter, improved sodium (Na+) exclusion and salinity tolerance in Arabidopsis. In the current work, AtHKT1;1, was expressed specifically in the root cortical and epidermal cells of an Arabidopsis GAL4-GFP enhancer trap line. These transgenic plants were found to have significantly improved Na+ exclusion under conditions of salinity stress. The feasibility of a similar biotechnological approach in crop plants was explored using a GAL4-GFP enhancer trap rice line to drive expression of AtHKT1;1 specifically in the root cortex. Compared with the background GAL4-GFP line, the rice plants expressing AtHKT1;1 had a higher fresh weight under salinity stress, which was related to a lower concentration of Na+ in the shoots. The root-to-shoot transport of 22Na+ was also decreased and was correlated with an upregulation of OsHKT1;5, the native transporter responsible for Na+ retrieval from the transpiration stream. Interestingly, in the transgenic Arabidopsis plants overexpressing AtHKT1;1 in the cortex and epidermis, the native AtHKT1;1 gene responsible for Na+ retrieval from the transpiration stream, was also upregulated. Extra Na+ retrieved from the xylem was stored in the outer root cells and was correlated with a significant increase in expression of the vacuolar pyrophosphatases (in Arabidopsis and rice) the activity of which would be necessary to move the additional stored Na+ into the vacuoles of these cells. This work presents an important step in the development of abiotic stress tolerance in crop plants via targeted changes in mineral transport. 2010 Plett et al.