Browsing by Author "Samir A."

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Antiviral potential of green synthesized silver nanoparticles of lampranthus coccineus and malephora lutea
(Dove Medical Press Ltd., 2019) Haggag E.G.; Elshamy A.M.; Rabeh M.A.; Gabr N.M.; Salem M.; Youssif K.A.; Samir A.; Bin Muhsinah A.; Alsayari A.; Abdelmohsen U.R.; Department of Pharmacognosy; Faculty of Pharmacy; Helwan University; Cairo; 11795; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Modern University for Technology and Information; Cairo; Egypt; Department of Pharmaceutical Chemistry; October University for Modern Sciences and Arts (Msa); Cairo; Egypt; Department of Pharmacognosy; College of Pharmacy; King Khalid University; Abha; 61441; Saudi Arabia; Department of Pharmacognosy; Faculty of Pharmacy; Minia University; Minia; Egypt
Background: Viral and microbial infections constitute one of the most important life-threatening problems. The emergence of new viral and bacterial infectious diseases increases the demand for new therapeutic drugs. Purpose: The objective of this study was to use the aqueous and hexane extracts of Lampranthus coccineus and Malephora lutea F. Aizoaceae for the synthesis of silver nanoparticles, and to investigate its possible antiviral activity. In addition to the investigation of the phytochemical composition of the crude methanolic extracts of the two plants through UPLC-MS metabolomic profiling, and it was followed by molecular docking in order to explore the chemical compounds that might contribute to the antiviral potential. Methods: The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy and Fourier transform infrared spectroscopy. The antiviral activity of the synthesized nanoparticles was evaluated using MTT assay against HSV-1, HAV-10 virus and Coxsackie B4 virus. Metabolomics profiling was performed using UPLC-MS and molecular docking was performed via Autodock4 and visualization was done using the Discovery studio. Results: The early signs of SNPs synthesis were detected by a color change from yellow to reddish brown color. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 10.12 nm to 27.89 nm, and 8.91 nm 14.48 nm for Lampranthus coccineus and Malephora lutea aqueous and hexane extracts respectively. The UV-Visible spectrophotometric analysis showed an absorption peak at ?max of 417 nm.The green synthesized SNPs of L. coccineus and M. lutea showed remarkable antiviral activity against HSV-1, HAV-10, and CoxB4 virus. Metabolomics profiling of the methanolic extract of L. coccineus and M. lutea resulted in identifying 12 compounds. The docking study predicted the patterns of interactions between the compounds of L. coccineus and M. lutea with herpes simplex thymidine kinase, hepatitis A 3c proteinase, and Coxsackievirus B4 3c protease, which was similar to those of the co-crystal inhibitors and this can provide a supposed explanation for the antiviral activity of the aqueous and nano extracts of L. coccineus and M. lutea. Conclusion: These results highlight that SNPs of L. coccineus and M. lutea could have antiviral activity against HSV-1, HAV-10, and CoxB4 virus. � 2019 Haggag et al. This work is published and licensed by Dove Medical Press Limited.
Development and validation of simultaneous spectrophotometric and TLC-spectrodensitometric methods for determination of beclomethasone dipropionate and salbutamol in combined dosage form
(Elsevier, 2014) Samir A.; Lotfy H.M.; Salem H.; Abdelkawy M.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts; Egypt
Spectrophotometric and TLC-spectrodensitometric methods were developed and validated for the simultaneous determination of beclomethasone dipropionate (BEC) and salbutamol (SAL). The spectrophotometric methods include dual wavelength, ratio difference, constant center coupled with a novel method namely, spectrum subtraction and mean centering with mean percentage recoveries and RSD 99.72 � 1.07 and 99.70 � 1.12, 100.25 � 1.12 and 99.89 � 1.12, 99.66 � 1.85 and 99.19 � 1.32, 100.74 � 1.26 and 101.06 � 0.90 for BEC and SAL respectively. The TLC-spectrodensitometric method was based on separation of both drugs on TLC aluminum plates of silica gel 60 F254, using benzene: methanol: triethylamine (10:1.5:0.5 v/v/v) as a mobile phase, followed by densitometric measurements of their bands at 230 nm. The mean percentage recoveries and RSD were 99.07 � 1.25 and 101.35 � 1.50 for BEC and SAL respectively. The proposed methods were validated according to ICH guidelines and were applied for the simultaneous analysis of the cited drugs in synthetic mixtures and pharmaceutical preparation. The methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of BEC and SAL in their pharmaceutical formulation with no need for prior separation. The results obtained were statistically compared to each other and to that of the reported HPLC method. The statistical comparison showed that there is no significant difference regarding both accuracy and precision. � 2014 Elsevier B.V. All rights reserved.
Spectrophotometric methods for simultaneous determination of betamethasone valerate and fusidic acid in their binary mixture
(Elsevier, 2015) Lotfy H.M.; Salem H.; Abdelkawy M.; Samir A.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr El-Aini Street; Cairo; 11562; Egypt; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; Deraya University; Minia; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; MSA University; 6th October City; Egypt
Five spectrophotometric methods were successfully developed and validated for the determination of betamethasone valerate and fusidic acid in their binary mixture. Those methods are isoabsorptive point method combined with the first derivative (ISO Point - D1) and the recently developed and well established methods namely ratio difference (RD) and constant center coupled with spectrum subtraction (CC) methods, in addition to derivative ratio (1DD) and mean centering of ratio spectra (MCR). New enrichment technique called spectrum addition technique was used instead of traditional spiking technique. The proposed spectrophotometric procedures do not require any separation steps. Accuracy, precision and linearity ranges of the proposed methods were determined and the specificity was assessed by analyzing synthetic mixtures of both drugs. They were applied to their pharmaceutical formulation and the results obtained were statistically compared to that of official methods. The statistical comparison showed that there is no significant difference between the proposed methods and the official ones regarding both accuracy and precision. � 2015 Elsevier B.V. All rights reserved.