Browsing by Author "Salem, M. Alaraby"

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Chemical and biological studies on the soft coral Nephthea sp
(Royal Society of Chemistry, 2021-06) Abdelhafez, Omnia Hesham; Fahim, John Refaat; El Masri, Ramy R.; Salem, M. Alaraby; Desoukey, Samar Yehia; Ahmed, Safwat; Kamel, Mohamed Salah; Pimentel-Elardo, Sheila Marie; Nodwell, Justin R; Abdelmohsen, Usama Ramadan
Soft corals belonging to the family Nephtheidae have been appreciated as marine sources of diverse metabolites with promising anticancer potential. In view of that, the current work investigates the anti- proliferative potential of the crude extract, different fractions, and green synthesized silver nanoparticles (AgNPs) of the Red Sea soft coral, Nephthea sp. against a panel of tumor cell lines. The metabolic pool of the soft coral under study was also explored via an LC-HR-ESI-MS metabolomics approach, followed by molecular docking analysis of the characterized metabolites against the target proteins, EGFR, VEGFR, and HER2 (erbB2) that are known to be involved in cancer cell proliferation, growth, and survival. Overall, the n- butanol fraction of Nephthea sp. exhibited the highest inhibitory activities against MCF7 (breast cancer) and A549 (lung cancer) cell lines, with interesting IC50 values of 2.30 0.07 and 3.12 0.10 mg ml 1 , respectively, whereas the maximum growth inhibition of HL60 (leukemia) cells was recorded by the total extract (IC50 ¼ 2.78 0.09 mg ml 1 ). More interestingly, the anti-proliferative potential of the total soft coral extract was evidently improved when packaged in the form of biogenic AgNPs, particularly against A549 and MCF7 tumor cells, showing IC50 values of 0.72 0.06 and 9.32 0.57 mg ml 1 , respectively. On the other hand, metabolic profiling of Nephthea sp. resulted in the annotation of structurally diverse terpenoids, some of which displayed considerable binding affinities and molecular interactions with the studied target proteins, suggesting their possible contribution to the anti-proliferative properties of Nephthea sp. via inhibition of tyrosine kinases, especially the EGFR type. Taken together, the present findings highlighted the relevance of Nephthea sp. to future anticancer drug discovery and provided a base for further work on the green synthesis of a range of bioactive NPs from marine soft corals.
Chemical and biological studies on the soft coral Nephthea sp.
(Royal Society of Chemistry, 2021-06) Abdelhafez, Omnia Hesham; Fahim, John Refaat; El Masri, Ramy R; Salem, M. Alaraby; Desoukey, Samar Yehia; Ahmed, Safwat; Kamel, Mohamed Salah; Pimentel-Elardo, Sheila Marie; Nodwell, Justin R; Abdelmohsen, Usama Ramadan
Soft corals belonging to the family Nephtheidae have been appreciated as marine sources of diverse metabolites with promising anticancer potential. In view of that, the current work investigates the anti- proliferative potential of the crude extract, different fractions, and green synthesized silver nanoparticles (AgNPs) of the Red Sea soft coral, Nephthea sp. against a panel of tumor cell lines. The metabolic pool of the soft coral under study was also explored via an LC-HR-ESI-MS metabolomics approach, followed by molecular docking analysis of the characterized metabolites against the target proteins, EGFR, VEGFR, and HER2 (erbB2) that are known to be involved in cancer cell proliferation, growth, and survival. Overall, the n- butanol fraction of Nephthea sp. exhibited the highest inhibitory activities against MCF7 (breast cancer) and A549 (lung cancer) cell lines, with interesting IC50 values of 2.30 0.07 and 3.12 0.10 mg ml 1 , respectively, whereas the maximum growth inhibition of HL60 (leukemia) cells was recorded by the total extract (IC50 ¼ 2.78 0.09 mg ml 1 ). More interestingly, the anti-proliferative potential of the total soft coral extract was evidently improved when packaged in the form of biogenic AgNPs, particularly against A549 and MCF7 tumor cells, showing IC50 values of 0.72 0.06 and 9.32 0.57 mg ml 1 , respectively. On the other hand, metabolic profiling of Nephthea sp. resulted in the annotation of structurally diverse terpenoids, some of which displayed considerable binding affinities and molecular interactions with the studied target proteins, suggesting their possible contribution to the anti-proliferative properties of Nephthea sp. via inhibition of tyrosine kinases, especially the EGFR type. Taken together, the present findings highlighted the relevance of Nephthea sp. to future anticancer drug discovery and provided a base for further work on the green synthesis of a range of bioactive NPs from marine soft corals.
Dereplication Analysis and Antitrypanosomal Potential of the Red Sea Sponge Amphimedon sp. Supported by Molecular Modelling
(Springer, 2020-03) Hisham Shady, Nourhan; Elfakharany, Zeinab; Salem, M. Alaraby; Ahmed, Safwat; Fouad, Mostafa A.; Salah Kamel, Mohamed; Krischke, Markus; Mueller, Martin J.; Abdelmohsen, Usama Ramadan
The present investigation was oriented to the discovery of chemical compounds from the Red Sea sponge Amphimedon sp., as a source of active agents against Trypanosoma brucei, the causal agent of human sleeping sickness. Dereplication analysis of the active fraction from Amphimedon sp. using liquid chromatography coupled with high-resolution mass spectrometry revealed the chemical richness of this sponge with diverse alkaloidal classes such as purine, manzamine, bis-piperidine, and pyridine. Activity-guided fractionation of the total extract showed the antitrypanosomal activity concentrated in the ethyl acetate fraction (IC50 = 3.8 μg/ml). In silico modelling was carried out on the dereplicated compounds to provide an insight into their antitrypanosomal mechanism of action with docking study on eight trypanosomal proteins. Molecular dynamics was run for the complex of zamamidine D and ornithine decarboxylase, which illustrated that zamamidine D has the highest affinity to the ornithine decarboxylase enzyme. These results highlight the valuable chemical profile of Amphimedon sp., as a lead source for antitrypanosomal natural products.