Browsing by Author "Saleh S."

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    Antifibrotic effects of gallic acid on hepatic stellate cells: In vitro and in vivo mechanistic study
    (National Taiwan University, 2019) El-Lakkany N.M.; El-Maadawy W.H.; Seif el-Din S.H.; Saleh S.; Safar M.M.; Ezzat, Shahira M; Mohamed S.H.; Botros S.S.; Demerdash Z.; Hammam O.A.; Department of Pharmacology; Theodor Bilharz Research Institute; Warak El-Hadar; Imbaba P.O. Box 30; Giza; 12411; Egypt; Department of Pharmacology and Toxicology; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Immunology; Theodor Bilharz Research Institute; Warak El-Hadar; Imbaba P.O. Box 30; Giza; 12411; Egypt; Department of Pathology; Theodor Bilharz Research Institute; Warak El-Hadar; Imbaba P.O. Box 30; Giza; 12411; Egypt; Department of Pharmacology and Biochemistry; Faculty of Pharmacy; The British University in Egypt; Suez Desert Road; P.O. Box 43; ElSherouk City; Cairo 11837; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th of October; Giza; 12566; Egypt
    Few studies reported the antifibrotic effects of gallic acid (GA) despite its known hepatoprotective and antioxidant activities. Accordingly, this study investigated the antifibrotic effects of GA through clarifying its mechanisms on hepatic stellate cells� (HSCs) activation, proliferation and/or apoptosis. In vitro effects of GA on HSC-T6 activation/proliferation, morphology and safety on hepatocytes were assessed. In vivo, hepatic fibrosis was induced via chronic thioacetamide (TAA)-intoxication. TAA-intoxicated rats were treated with silyamrin or GA. At end of experiment, liver functions, hepatic MDA, GSH, PDGF-BB, TGF-?1, TIMP-1 and hydroxyproline were determined. Histological analysis and Sirius red staining of hepatic sections, expressions of alpha-smooth muscle actin (?-SMA), proliferating cellular nuclear antigen (PCNA) and caspase-3 were examined. In vitro, GA resulted in a concentration and time-dependent inhibition in HSCs activation, proliferation (IC50= 45 and 19 ?g/mL at 24 and 48 h respectively); restored the quiescent morphology of some activated HSCs plus its safety on hepatocytes. In vivo, GA reduced ALT, AST, MDA, PDGF-BB levels, collagen deposition and fibrosis score (S1 vs S4); increased caspase-3 expression and restored GSH stores, TGF-?1 level, ?-SMA and PCNA expressions. In conclusion, GA counteracted the progression of hepatic fibrosis through reduction of HSCs proliferation/activation mutually with their apoptosis induction. � 2018 Center for Food and Biomolecules, National Taiwan University
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    Comparative study of RP-HPLC versus TLC-spectrodensitometric methods applied for binary mixtures of fluoroquinolones and corticosteroids
    (Akademiai Kiado Rt., 2014) Elgizawy S.; Hassan N.; Lotfy H.; Saleh S.; Assuit University; Analytical Chemistry Department; Faculty of Pharmacy; 71515 Assuit; Egypt; Cairo University Analytical Chemistry Department; Faculty of Pharmacy; Kasr-El Aini Street; 11562 Cairo; Egypt; October University for Modern Sciences and Arts (MSA); Analytical Chemistry Department; Faculty of Pharmacy; 11787 6th October City; Egypt
    Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-spectrodensitometric methods have been developed and validated for the separation and quantitation of two binary mixtures: Ofloxacin (OFX) and dexamethasone (DXM) in eye preparation; ciprofloxacin hydrochloride (CIP) and hydrocortisone (HYD) in ear preparation. The linearity ranges of RP-HPLC methods were found to be (2.5-45 ?g mL-1) for OFX, (2.5-50 ?g mL-1) for DXM and (1-8 ?g mL-1) for both CIP and HYD. The percentage recoveries/relative standard deviation (RSD) were found to be 100.36/1.38, 100.13/1.49, 99.98/0.61 and 100.28/1.27, respectively. The linearity ranges of TLC-spectrodensitometric methods were found to be (0.5-2 ?g band-1), (0.5-3.5 ?g band-1), (0.2-1.6 ?g band-1), and (0.6-2 ?g band-1) for OFX, DXM, CIP, and HYD, respectively. The percentage recoveries/RSD were found to be 99.98/1.06, 99.93/1.18, 99.74/1.27, and 99.94/1.54, respectively. A comparative study was conducted to show the advantages of the proposed methods which showed that the TLC-spectrodensitometric methods were simpler, more sensitive, and economic, while RP-HPLC methods were more precise and robust. The methods were validated in compliance with the ICH guidelines and were successfully applied for determination of the selected drugs in their laboratory-prepared mixtures and commercial dosage forms.