Browsing by Author "Mousa, Mohamed R"

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Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats
(PeerJ Inc, 2023-12) El-Shehry, Mona S. E. F; Amrymi, Rafa A; Atia, Tarek; Lotfy, Bassant M. M; Ahmed, Salma H. A; Qutb, Sarah A; Ali, Sara B; Mohamed, Ayman S; Mousa, Mohamed R; Damanhory, Ahmed A; Metawee, Mostafa E; Sakr, Hader I
Background: Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes’ average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins. Aim: Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)- induced HA in rats. Methods: Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days. Results: Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate & alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde & nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement. Conclusion: Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.
Impact of different processing methods on the phenolics and neuroprotective activity of Fragaria ananassa Duch. extracts in a D-galactose and aluminum chloride-induced rat model of aging
(Royal Society of Chemistry, 2022-07) Ezzat, Marwa I; Issa, Marwa Y; Sallam, Ibrahim E; Zaafar, Dalia; Khalil, Heba M. A; Mousa, Mohamed R; Sabry, Dina; Gawish, Aya Y; Elghandour, Ahmed H; Mohsen, Engy
Age-related diseases, including dementia, are a major health concern affecting daily human life. Strawberry (Fragaria ananassa Duch.) is the most eaten fruit worldwide due to its exceptional aroma and flavor. However, it’s rapid softening and decay limit its shelf-life. Freezing and boiling represent the well- known conservation methods to extend its shelf-life. Therefore, we aimed to discover the phytochemical content differences of fresh and processed strawberries associated with investigating and comparing their neuroprotective effects in a rat model of aging. Female Wistar rats were orally pretreated with fresh, boiled, and frozen F. ananassa methanolic extracts (250 mg kg−1 ) for 2 weeks, and then these extracts were concomitantly exposed to D-galactose [65 mg kg−1 , subcutaneously (S/C)] and AlCl3 (200 mg kg−1 , orally) for 6 weeks to develop aging-like symptoms. The results of UPLC/ESI-MS phytochemical profiling revealed 36 secondary metabolites, including phenolics, flavonoids, and their glycoside derivatives. Compared with boiled and frozen extracts, the fresh extract ameliorated the behavioral deficits including anxiety and cognitive dysfunction, upregulated brain HO-1 and Nrf2 levels, and markedly reduced caspase-3 and PPAR-γ levels. Moreover, LDH and miRNA-9, 124 and 132 protein expressions were reduced. The histological architecture of the brain hippocampus was restored and glial fibrillary acidic protein (GFAP) immunoexpression was downregulated. In conclusion, the fresh extract has neuroprotec- tive activity that could have a promising role in ameliorating age-related neurodegeneration.
Therapeutic potency of gallium verum extract on ethanol-induced gastric ulcer in rats
(AMG Transcend Association, 15/10/2022) Lotfy, Bassant Mohamed Mohamed; Mousa, Mohamed R; El-Shehry, Mona Samir Fawzy El-Sayed; Ahmed, Salma Hussein Abdel-Fattah; Ali, Sara Bayoumi; Al Shawoush, Abdeljalil Mohamed; Mohamed, Ayman Saber
Gallium verum, lady's bedstraw is an herbaceous annual plant belonging to the genus Rubiaceae; it possessed antioxidant, cytotoxic for cancer cells, antimicrobial, protective and endocrine effects. This work aimed to investigate the curative effects of G. verum extract on gastric ulcers following absolute ethanol administration in the healthy rat. Eighteen rats were randomly divided into three main groups; rats were fastened for 24 hours before ethanol administration. All groups except control administered ethanol (5 ml/kg body weight; orally). The rats were administrated distilled water (Ulcer group) or G. verum extract (100mg/kg) one hour later. G. verum extract caused a significant decrease in ulcer index, gastric juice volume, malondialdehyde, and nitric oxide, while gastric juice pH, glutathione, glutathione-S-transferase, and catalase increased significantly. The histological lesion score showed a significant enhancement in group G. verum compared to the ulcer group that scored the highest pathological destruction score. Immunohistochemical markers of NF-κB p65 and TNF-α showed a significant decrease in G. verum group. G. verum extract is a promising treatment modality against gastric injury through its powerful antioxidant, acid neutralizing, healing promotion, and ant- inflammation effects.