Browsing by Author "M. Riad, Safaa"

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Analytical Investigation on Green Smart Spectrophotometric Methods Utilizing Unified Regression Equation
(Taylor & Francis, 2018) S. Saleh, Sarah; M. Riad, Safaa
Three green smart spectrophotometric methods namely; absorbance subtraction (AS), amplitude modulation (AM) and amplitude summation (A-Sum) were developed and validated for the determination of a binary mixture of chlorzoxazone (CHZ) and ibuprofen (IBU) without prior separation, using unified regression equation. The proposed spectrophotometric procedures were found to be linear in the range of (2.5-27.5 μg/ml) and (2-22 μg/ml) for CHZ and IBU, respectively. The methods were validated as per ICH guidelines and the results were found to be within the acceptable limits. The specificity was assessed by analyzing synthetic mixtures of both drugs which showed the superiority of AM and A-Sum methods over AS method in the analysis of low concentrations. The proposed methods were applied to pharmaceutical formulations and the results were statistically compared to that of a reported spectrophotometric method. The statistical comparison showed that there is no significant difference among the methods regarding both accuracy and precision.
Electrochemical determination of ciprofloxacin hydrochloride in pharmaceutical formulation, aquatic environ-ment and in fish tissues
(Int. J. Biol. Pharm. Res, 2013) R. Rezk, Mamdouh; M. Riad, Safaa; I. Khattab, Fatma; M. Marzouk, Hoda
Two ciprofloxacin hydrochloride (CPX) selective electrodes were developed using dioctyl phthalate (DOP) as a plasticizer in a polymeric matrix of polyvinyl chloride (PVC). Sensor I was fabricated using tetrakis(4-chlorophenyl)borate (TpClPB) as an anionic exchanger without incorporation of an ionophore. Sensor II was developed using 2-hydroxy propyl β-cyclodextrin as an ionophore. Linear responses of CPX within a concentration range of 10-5-10-2 M, with slopes of 51.7 ± 0.31 and 48.7 ± 0.40 mV/ decade over pH range of 4-7 were obtained using sensors I and II, respectively. The selectivity coefficients of the developed sensors indicated excellent selectivity for CPX. The proposed method displayed useful analytical characteristics for determination of ciprofloxacin hydrochloride in pharmaceutical formulation , fish water and fish tissues with average recoveries of 100.91 ± 0.47, 96.42 ± 1.34 and 86.46 ± 1.28 , respectively for sensor I and 101.2 ± 0.37 , 97.26± 1.56 and 86.67± 1.70 , respectively for sensor II.
Simultaneous determination of metronidazole and diiodohydroxyquine in bulk powder and paramibe compound tablets by TLC-densitometry and HPLC
(Pharmaceut Anal Acta, 2012) Salem, Hesham; M. Riad, Safaa; R. Rezk, Mamdouh; Ahmed, Kholoud
Two sensitive and precise chromatographic methods were developed and validated for simultaneous determination of metronidazole (MTR) and diiodohydroxyquin (DIQ) in pharmaceutical preparation. The techniques adopted for quantification are coupled TLC-densitometry and HPLC. A mixture of chloroform, toluene, ethanol and acetic acid (9:9:1:1, v/v/v/v) was used as the developing solvent for TLC-densitometry. A mixture of methanol and acetonitrile, (96:4, v/v) was used as a mobile phase for HPLC at 0.6 mL min-1 flow rate and UV detection at 254 nm. Linearity was obtained in concentration range of 0.5-10 µg spot-1 for DIQ and 1-20 µg spot-1 for MTR applying TLC-densitometry and 0.005-0.5 mg mL-1 for DIQ and 0.01-0.5 mg mL-1 for MTR applying HPLC. The selectivity of the proposed methods was checked using laboratory prepared mixtures. The proposed methods were successfully applied to the analysis of MTR and DIQ in their mixture and in pharmaceutical dosage forms without interference from other additives.
Spectrophotometric determination of sitagliptin and metformin in their pharmaceutical formulation
(Pharmacie Globale, 2012) M. Riad, Safaa; R. Rezk, Mamdouh; Y. Mahmoud, Ghada; El Bayoumi Abdel Aleem, Abdel-Aziz
Two simple, accurate and precise spectrophotometric methods were developed and validated for the determination of sitagliptin phosphate monohydrate (STA) and metformin hydrochloride (MTF). The first method was based on measuring the absorbance of STA at 268 nm in the range of 25-500 µg mL-1. The second method was the isosbestic point method. The total mixture concentration was calculated by measuring the absorbance at 257 nm. The proposed methods used to determine each drug in binary mixture. The results were statistically compared using one-way analysis of variance (ANOVA). The developed methods were satisfactory applied to the analysis of the pharmaceutical formulation and proved to be selective and accurate for the quality control of the cited drugs in their pharmaceutical formulation.
Stability indicating spectrophotometric and spectrodensitometric methods for the determination of diatrizoate sodium in presence of its degradation product
(Elsevier, 2015) K. Abd El-Rahman, Mohamed; M. Riad, Safaa; A. Abdel Gawad, Sherif; M. Fawaz, Esraa; A. Shehata, Mostafa
Three sensitive, selective, and precise stability indicating methods for the determination of the X-ray contrast agent, diatrizoate sodium (DTA), in the presence of its acidic degradation product (highly cytotoxic 3,5 diamino metabolite) and in pharmaceutical formulation were developed and validated. The first method is a first derivative (D1) spectrophotometric one, which allows the determination of DTA in the presence of its degradate at 231.2 nm (corresponding to zero crossing of the degradate) over a concentration range of 2–24 lg/mL with mean percentage recovery 99.95 ± 0.97%. The second method is the first derivative of the ratio spectra (DD1) by measuring the peak amplitude at 227 nm over the same concentration range as D1 spectrophotometric method, with mean percentage recovery 99.99 ± 1.15%. The third method is a TLC-densitometric one, where DTA was separated from its degradate on silica gel plates using chloroform:methanol:ammonium hydroxide (20:10:2 by volume) as a developing system. This method depends on quantitative densitometric evaluation of thin layer chromatogram of DTA at 238 nm over a concentration range of 4–20 lg/spot, with mean percentage recovery 99.88 ± 0.89%. The selectivity of the proposed methods was tested using laboratory-prepared mixtures. The proposed methods have been successfully applied to the analysis of DTA in pharmaceutical dosage forms without interference from other dosage form additives. The results were statistically compared with the official US pharmacopeial method. No significant difference for either accuracy or precision was observed.