Browsing by Author "Hegazy, Maha A"

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Greenness evaluation of different chromatographic approaches for the determination of dextromethorphan, phenylephrine & brompheniramine in their pharmaceutical formulation
(Elsevier Inc., 2020-09) Mohamed, Dalia; Hegazy, Maha A; El-Sayed, Ghada M; Youssef, Souha H.
View references (19) The effect of analytical procedures on the environment is a major concern nowadays. Scientists are trying to contribute to eco-friendly practices to reduce the negative impact of chemicals and solvents on the environment and analysts working on the field. This work demonstrates three chromatographic methods for the determination of a cold medication composed of dextromethorphan (DEX), phenylephrine (PHN) and brompheniramine (BRM) in their dosage form. Thin layer chromatography (TLC) was attempted using methanol: methylene chloride: water: acetic acid (7:1:1.5:0.5, v/v/v/v) as a mobile phase and detection carried out at 254 nm. The drugs were separated with good resolution at retention factors 0.54, 0.66 and 0.78 for BRM, DEX and PHN, respectively. Two high performance liquid chromatographic (HPLC) methods were also applied; method A utilized a mobile phase made up of acetonitrile, phosphate buffer and methanol (gradient elution) and green solvents were applied in method B namely; ethanol: water (15:85, v/v). Peaks were detected at 270 nm and the retention times for method A were 2.02, 7.59 and 8.43 min and for method B were 2.04, 4.25 and 4.91 min for PHN, BRM and DEX, respectively. Greenness profiles using three approaches; national environmental method index (NEMI), analytical ecoscale and green analytical procedure index (GAPI) were used to evaluate and compare the developed methods. Finally, statistical analysis was applied and no significant difference regarding accuracy and precision was noted
Portable Nanoparticle-Enhanced Sensor for Tigecycline Determination in Biological Fluids and in the Presence of Its Degradation Products
(Electrochemical Society, Inc., 2023-08) Abd El-Fatah, Nourhan A; El-Sayed, Ghada M; Hegazy, Maha A; Fouad, Manal Mohammed; Elbalkiny, Heba T
Tigecycline (TGC) is a novel potent antibiotic with recently proven anticancer activity against leukemia, glioma, and lung cancer. In-line TGC potentiometric sensors are fabricated for monitoring TGC in its pure form, pharmaceutical formulation, presence of its degradation products, and spiked human plasma. In-line sensors act as greener, portable, and economical alternatives to the classical off-line separation-based techniques. Classical and advanced liquid-contact (LC) and solid-contact (SC) sensors were fabricated, where the best performance was observed with the modified SC sensor (sensor VI) with potassium tetrakis (4- chlorophenyl) borate as ionic exchanger, β-cyclodextrin ionophore and cobalt oxide nanoparticles, showing a Nernstian response of 30 mV decade−1 in the linear range of 10−2 –10−6 M. Statistical comparison was carried out for the results obtained from proposed SC sensors and the official method on TGC pure form. Additionally, method greenness was evaluated using a semi-quantitative analytical eco-scale, scoring approximately 95 points, which was the highest greenness achievement score when compared to the proposed LC sensors or British Pharmacopeial chromatographic method.