Browsing by Author "Hassouna N."

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    Antagonistic activity of lactobacillus isolates against salmonella typhi in vitro
    (2013) Abdel-Daim A.; Hassouna N.; Hafez M.; Ashor M.S.A.; Aboulwafa M.M.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo 12611; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Ain Shams University; Al Khalifa Al Maamoun Street; Abbassia; Cairo 11566; Egypt; Department of Pharmaceutical Microbiology; College of Pharmacy; Taif University; Saudi Arabia
    Background. Enteric fever is a global health problem, and rapidly developing resistance to various drugs makes the situation more alarming. The potential use of Lactobacillus to control typhoid fever represents a promising approach, as it may exert protective actions through various mechanisms. Methods. In this study, the probiotic potential and antagonistic activities of 32 Lactobacillus isolates against Salmonella typhi were evaluated. The antimicrobial activity of cell free supernatants of Lactobacillus isolates, interference of Lactobacillus isolates with the Salmonella adherence and invasion, cytoprotective effect of Lactobacillus isolates, and possibility of concurrent use of tested Lactobacillus isolates and antibiotics were evaluated by testing their susceptibilities to antimicrobial agents, and their oxygen tolerance was also examined. Results. The results revealed that twelve Lactobacillus isolates could protect against Salmonella typhi infection through interference with both its growth and its virulence properties, such as adherence, invasion, and cytotoxicity. These Lactobacillus isolates exhibited MIC values for ciprofloxacin higher than those of Salmonella typhi and oxygen tolerance and were identified as Lactobacillus plantarum. Conclusion. The tested Lactobacillus plantarum isolates can be introduced as potential novel candidates that have to be subjected for in vivo and application studies for treatment and control of typhoid fever. � 2013 Amira Abdel-Daim et al.
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    Screening of Lactobacillus isolates for their adherence capabilities to mammalian cells and their acid and bile tolerance
    (2012) Abdel-Daim A.; Hassouna N.; Hafez M.; Ashor M.S.A.; Aboulwafa M.M.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Department of Pharmaceutical Microbiology; College of Pharmacy; Taif University; Saudi Arabia
    Background: Lactobacilli resemble a major part of the commensal human mucosal flora. The application of Lactobacilli as probiotics has increased during the last few years since a health promoting effect has been reported in addition to their long history of safe use. Methods and finding: In this study, fifty-two Lactobacillus isolates were recovered from dairy products or infant stools were examined in vitro for their probiotic potential. Their adherence capacities to Vero cells in addition to their acid and bile tolerance were evaluated. Only few isolates weren't able to adhere to Vero cells, while the other isolates have high to moderate adherence capacities. The majority of isolates were tolerant to acid and about 70% were tolerant to 0.3% bile salts. Conclusion: thirty two Lactobacillus isolates were found to possess desirable probiotic properties. These isolates are good candidates for further investigation in in vitro and in vivo studies for their potential health benefits and their application as novel Biotherapeutic agents. � iMedPub.