Browsing by Author "Hashad I.M."

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Assessment of the link between endothelin K198n Snp, endothelin concentration and acute myocardial infarction in Egyptians
(Blackwell Publishing, 2017) Abdel Rahman M.F.; Hashad I.M.; Abou-Aisha K.; Abdel Maksoud S.M.; Gad M.Z.; Biochemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Cairo; Egypt; Clinical Biochemistry Unit; Faculty of Pharmacy & Biotechnology; German University in Cairo; Cairo; Egypt
The aim of the current study was to assess the link between EDN K198N SNP, ET-1 serum concentration and acute myocardial infarction (AMI) in Egyptians. The study cohort consisted of 84 patients at AMI onset and 84 age-matched healthy controls. Endothelin genotypes and concentrations were determined by sequencing and ELISA, respectively. Genotype distribution was not significantly different between AMI patients and controls (P=.8341). The mean serum ET-1 concentration of patients (13.83�0.7�pg/mL) was significantly higher than controls (7.26�0.2�pg/mL) (P<.0001). ET-1 serum concentrations did not vary significantly among various EDN genotypes in patients (P=.378) and controls (P=.6164). Hence, we conclude that EDN K198N genotypes were not related to either ET-1 concentration or incidence of early-onset AMI in Egyptians. But, AMI patients had higher ET-1 concentrations than controls. � 2016 John Wiley & Sons Australia, Ltd
The association of fractalkine receptor (T280m) polymorphism in the pathogenesis of acute coronary syndrome in the Egyptian population
(Bentham Science Publishers B.V., 2019) Hassan D.S.; Hashad I.M.; Rahman M.F.A.; Abdel-Maksoud S.M.; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; German University in Cairo; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Science and Arts; Giza; Egypt
Fractalkine (FKN) in its free and membrane bound-forms and its receptor CX3CR1are reported to have an atherosclerotic effect. The relationship of Single Nucleotide Polymorphisms (SNPs) in FKN and CX3CR1genes with the Coronary Artery Disease (CAD) risk showed conflicting results in different populations. The aim of this study was to investigate the influence of CX3CR1 threonine 280 methionine (T280M) polymorphism in the predisposition of Acute Coronary Syndrome (ACS) in Egyptians. Methods: 200 Egyptian subjects were recruited for the study. They were divided into 100 ACS patients and 100 healthy controls. Genotyping of CX3CR1 T280M was performed using a Polymerase Chain Reaction-restriction Fragment Length Polymorphism (PCR-RFLP). Serum FKN was assayed by Enzyme - Linked - Immuno- Sorbent-Assay (ELISA). Results: T and M allele frequencies for CX3CR1gene were not significantly different between ACS and Controls (p=0.76). Moreover, none of the genotypes had an atheroprotective effect. Serum analysis showed higher levels of FKN in ACS patients (p=0.041). FKN levels were not significantly different among genotypes of control and ACS groups (p=0.34) and (p=0.38) respectively. Conclusion: This study shows that CX3CR1 T280M polymorphism does not affect the incidence of ACS the Egyptian population. Moreover, none of the genotypes were associated with higher FKN levels. � 2018 Bentham Science Publishers.
Association of manganese superoxide dismutase Ala16Val polymorphism in the incidence of acute myocardial infarction in the Egyptians
(Academy of Scientific Research and Technology, 2017) Abdelrauf L.M.; Abdel Rahman M.F.; Abdel-Maksoud S.M.; Farag N.M.; Hashad I.M.; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; The German University in Cairo; Egypt; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; Egypt; Department of Cardiology; Faculty of Medicine; Ain Shams University; Cairo; Egypt
Background: Oxidative stress has been implicated in various diseases including atherosclerosis; the most common pathologic process underlying acute myocardial infarction (AMI). The manganese superoxide dismutase (MnSOD) antioxidant enzyme affords the major defense against reactive oxygen species (ROS) within the mitochondria. MnSOD Alanine16Valine (A16V) single nucleotide polymorphism (SNP) has been shown to decrease MnSOD detoxification activity. Aim: A case-control study was conducted to investigate the association between MnSOD A16V polymorphism and the incidence of AMI in the Egyptians, investigate the contribution of oxidative stress represented by hexanoyl lysine adduct (HEL), an oxidative stress biomarker, in the pathogenesis of AMI and finally correlate the MnSOD genotypes with HEL serum levels. Methods: A total of 200 Egyptian subjects were recruited for the study; 100 AMI patients and 100 control subjects. Genotypes of the MnSOD A16V polymorphism were determined using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum HEL was measured by ELISA. Results: A significant difference in the distribution of the MnSOD A16V genotypes was observed; VV genotype was significantly higher in AMI than controls (p ? 0.0001). Also, studying the allele frequencies revealed that Val allele was significantly higher in AMI than controls (p ? 0.0001). Serum analysis showed higher levels of HEL in AMI patients (p = 0.0142). Furthermore, HEL levels were found to be significantly higher in VV genotype in AMI (p = 0.0273). Conclusions: Our study suggests that MnSOD A16V polymorphism is associated with increased risk of developing AMI in the Egyptians. Moreover, the VV genotype is associated with higher HEL levels. � 2017
C(-260)T polymorphism in CD14 receptor gene of Egyptians with acute myocardial infarction
(Bentham Science Publishers B.V., 2018) Hashad I.M.; Hossni N.M.; Abdel Rahman M.F.; Shehata M.; Shaban G.; Gad M.Z.; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; The German University in Cairo; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Cairo; Egypt; Department of Cardiology; Faculty of Medicine; Ain Shams University; Cairo; Egypt; Department of Cardiology; National Heart Institute; Cairo; Egypt
Background: Despite the significance of the traditional risk factors, recently published studies have suggested that inflammatory processes and variations in the genetics of the inflammatory system may participate in the initiation of atherosclerosis and its complications. Objective: To investigate the possible association between CD14 C(-260)T (rs2569190) gene polymorphism and the risk of acute myocardial infarction in the Egyptian population. Methods: We enrolled 100 acute myocardial infarction patients in addition to 107 healthy controls. Deoxyribonucleic acid was extracted, purified and used for the genotype assay of C(-260)T polymorphism in promoter region of CD14 gene. Genotyping was conducted using polymerase chain reaction-restriction fragment length polymorphism. Polymerase chain reaction product was digested using a restriction enzyme and the digestion products were specified. Serum CD14 levels were determined by Enzyme Linked Immunosorbent Assay. Results: CD14 genotypic distribution (CC: 15.9% vs. 16%, CT: 62.6% vs. 58%, TT: 21.5% vs. 26% in controls versus acute myocardial infarction patients, p > 0.05 for all variables) and allele frequencies (C allele: 47% vs., 45%, T allele: 52% vs. 55% in controls versus acute myocardial infarction patients, p > 0.05 for all variables) did not show a statistical significant difference. Serum CD14 levels were elevated in acute myocardial infarction patients (5.73�0.62 vs. 4.48�0.28 pg/ml, p < 0.05). However, there was no statistically significant difference in serum CD14 levels among different CD14 genotypes. Conclusion: CD14 C-(260)T polymorphism is not associated with incidence of acute myocardial infarction in Egyptians who showed elevated serum CD14 levels in comparison to healthy individuals. � 2018 Bentham Science Publishers.
Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of keap-1/nrf-2 pathway.
(Elsevier B.V., 2019) Saleh D.O.; Mansour D.F.; Hashad I.M.; Bakeer R.M.; Department of Pharmacology; Medical Research Division; The National Research Centre; 33 EL Bohouth St. (former EL Tahrir St.); P.O. 12622; Dokki; Giza; Egypt; Department of Clinical Pharmacy and Pharmacy Practice; Faculty of Pharmacy; Ahram Canadian University; Egypt; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; German University in Cairo; Cairo; Egypt; Department of Pathology; Faculty of Medicine; Helwan University; Egypt; Instructor of Pathology; October University of Modern Sciences and Arts (MSA)University; Egypt
Aging; a biological phenomenon characterized by progressive decline in cellular functions, is considered as a major risk factor of various liver diseases that plays as an adverse prognostic role, thus increasing mortality rate. However, diet is the main environmental factor that has a major impact on the aging process whereas; sulforaphane (SFN), an isothiocyanate organosulfur compound in cruciferous vegetables, has been reported with myriad biological effects. In the present study, SFN antiaging properties were evaluated on D-galactose (D-Gal)-induced liver aging in rats. For this purpose, forty adult male Wistar rats were divided into five groups. All animals, except the normal control, were intraperitoneally injected with D-Gal (300 mg/kg/day for 5 days a week)for six consecutive weeks. In the hepatoprotective groups, animals received oral SFN (0.5, 1.0 and 2.0 mg/kg)for 6 weeks concurrently with D-GAL. SFN administration improved liver biomarkers through decreasing serum levels of AST, ALT, total and direct bilirubin when compared to D-Gal-aging group. SFN significantly increased hepatic GSH level as well as catalase and glutathione-S-transferase activities while counteracted the elevation in hepatic oxidative stress markers; MDA, NO and protein carbonyl in aged rats. SFN abrogated the dysregulation in hepatic Keap-1, Nrf-2 and HO-1and limited the elevation of TNF-? and TGF-? concentrations in aging liver. Histopathologically, SFN decreased the intensity of hepatic fibrous proliferation in D-Gal-induced aging. In conclusion, SFN has shown hepatic anti-aging potential through promoting the antioxidant machinery via regulating Keap-1, Nrf-2 and HO-1 and antioxidant enzyme activities as well as ameliorating oxidative stress, hampering the inflammatory cytokines; TNF-? and TGF-?, and limiting hepatic fibrosis in a dose dependent manner. � 2019 Elsevier B.V.
Investigating the link between MCP-1 A-2518G, RANTES G-403A, CX3CR1 V249I and MTHFR C677T gene polymorphisms and the risk of acute myocardial infarction among Egyptians
(Elsevier B.V., 2017) Hashad I.M.; Abdel Rahman M.F.; Alenina N.; Bader M.; Gad M.Z.; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; German University in Cairo (GUC); New Cairo City; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); 6th of October City; Egypt; Cardiovascular Department; Max-Delbr�ck-Center for Molecular Medicine; Berlin-Buch; Germany
Background Acute myocardial infarction (AMI) is one of the leading causes of death among Egyptians. Monocyte chemoattractant protein-1 (MCP-1), regulation on activation normal T cell expressed and secreted (RANTES) and fractalkine (FKN) are chemokines that act as components of inflammatory response while methylenetetrahydrofolate reductase (MTHFR) is important enzyme in folate metabolism essential for homocysteine metabolism. Hyperhomocysteinemia has been linked to AMI. MCP-1 A-2518G, RANTES G-403A, CX3CR1 V249I and MTHFR C677T are important polymorphisms identified in MCP-1, RANTES, CX3CR1 and MTHFR genes respectively. There are conflicting data in the literature about their association with AMI. Therefore, the aim of the current study was to investigate the contribution of these gene variants to risk of AMI among Egyptians. Subjects and methods The study comprised 200 subjects; 100 AMI patients and 100 age-matched healthy controls. The MCP-1, RANTES, CX3CR1 and MTHFR genotypes were determined by restriction fragment length polymorphism (PCR-RFLP). Results Genotypes distributions for RANTES, fractalkine and MTHFR genes were significantly different between AMI patients and controls (p�=�0.0221, 0.0498 and 0.0083) while those results in MCP-1 were not significantly different. A significant risk for AMI with concurrent presence of RANTES (AG/AA), fractalkine (VV) and MTHFR (CT/TT) genotypes was observed. Conclusions 1 - Each of MTHFR 677T, RANTES-403A and CX3CR1 249V alleles is considered an independent risk factor for AMI. 2 - Concurrent presence of high risk genotypes of RANTES (AG/AA), fractalkine (VV) and MTHFR (CT/TT) increases risk of AMI more than their individual risks. 3 - MCP-1 polymorphism is not associated with AMI among Egyptians. � 2016 Elsevier B.V.
Polymorphisms in gap junction proteins and their role in predisposition of acute myocardial infarction in Egyptians
(Bentham Science Publishers B.V., 2017) El Tahry F.A.; Hashad I.M.; Rahman M.F.A.; Gad M.Z.; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; German University in Cairo; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Science and Arts; 6th of October City; Egypt
Background: Connexin (Cx) proteins are the building blocks of gap junctions. Among these, Cx37 and Cx40 are expressed on vascular system and reported to have cardioprotective role. Linking polymorphisms in genes coding for Cx and coronary artery disease (CAD) risk showed conflicting results in different populations. None has been studied before in Egyptians. Therefore, the aims of this study were to investigate the influence of Cx37 C1019T and Cx40 A71G polymorphisms on the predisposition of acute myocardial infarction (AMI) in Egyptians, to study linkage disequilibrium (LD) and combined effects of single nucleotide polymorphisms (SNPs) and to correlate the genotypes with sVCAM-1 serum levels. Methods: Total of 201 Egyptian subjects were recruited for the study. They were divided into 104 AMI patients and 97 healthy controls. Genotypes for each participant were determined using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Serum sVCAM-1was measured by ELISA. Results: Allele frequencies for both Cx37 and Cx40 were not significantly different between AMI and Controls (p=0.93 and p=0.26 respectively). Moreover, studying the dominant and recessive models concluded that none of the genotypes was a risk factor. Both SNPs were not in LD (R2=0.0027). Serum analysis showed higher levels of sVCAM-1 in AMI patients (p<0.0001). sVCAM-1 levels were not significantly different among SNPs (Cx37; p=0.244 and Cx40; p=0.266). � 2017 Bentham Science Publishers.