Browsing by Author "Hafez, Mohamed M"

Filter results by typing the first few letters
Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Association between Cluster of Differentiation 24 (CD24) Polymorphism, Talin-1 gene expression and Hepatocellular Carcinoma prevalence in Egyptian population
    (9/17/2021) Hafez, Mohamed M; Abdel-Hafez, Lina Jamil M; Jamil, Lubna; Ibrahim, Sherine M
    Background: Hepatitis C is considered one of the most popular diseases in Egypt. We aim is to clarify the association between Cluster of Differentiation 24 (CD24) polymorphism, Talin-1 gene expression, and the prevalence of hepatocellular carcinoma in Egyptian Hepatitis C virus patients. Methods: The link between CD24 polymorphism rs8734 and the prevalence of hepatocellular carcinoma was assessed between 200 control subjects and 400 hepatitis C virus patients (HCV), patients were classified as follows; 200 patients with HCV and 200 with HCV and hepatocellular carcinoma (HCC) by histopathological assessment and PCR-restriction fragment length polymorphism (PCR-RFLP). Results: The hepatitis c patients with HCC showed a significant increase in alpha - fetoprotein (AFP) and Talin-1 gene expression compared to patients with HCV as well as in healthy volunteers. Furthermore, the frequencies of CD 24 170 CT/TT genotype were significantly higher in HCV patients without complications (60%) when compared to CC genotype (40%) (OR= 6 at X2= 14.41, P = 0.0007), and in HCV with HCC patients (90%) when compared to CC genotype (10%) (OR= 36 at X2= 14.41, P = 0.0007). Conclusion: These data suggest that CD24 genetic polymorphism rs8734 and Talin-1 gene expression may be a significant determinant for the prevalence of hepatocellular carcinoma in HCV patients.
  • Thumbnail Image
    Item
    Association between Cluster of Differentiation 24 (CD24) Polymorphism, Talin-1 gene expression and Hepatocellular Carcinoma prevalence in Egyptian population
    (Bentus, 8/13/2021) Hafez, Mohamed M; Abdel-Hafez, Lina Jamil M; Jamil, Lubna; Ibrahim, Sherine M
    Background: Hepatitis C is considered one of the most popular diseases in Egypt. We aim is to clarify the association between Cluster of Differentiation 24 (CD24) polymorphism, Talin-1 gene expression, and the prevalence of hepatocellular carcinoma in Egyptian Hepatitis C virus patients. Methods: The link between CD24 polymorphism rs8734 and the prevalence of hepatocellular carcinoma was assessed between 200 control subjects and 400 hepatitis C virus patients (HCV), patients were classified as follows; 200 patients with HCV and 200 with HCV and hepatocellular carcinoma (HCC) by histopathological assessment and PCR-restriction fragment length polymorphism (PCR-RFLP). Results: The hepatitis c patients with HCC showed a significant increase in alpha - fetoprotein (AFP) and Talin-1 gene expression compared to patients with HCV as well as in healthy volunteers. Furthermore, the frequencies of CD 24 170 CT/TT genotype were significantly higher in HCV patients without complications (60%) when compared to CC genotype (40%) (OR= 6 at X2= 14.41, P = 0.0007), and in HCV with HCC patients (90%) when compared to CC genotype (10%) (OR= 36 at X2= 14.41, P = 0.0007). Conclusion: These data suggest that CD24 genetic polymorphism rs8734 and Talin-1 gene expression may be a significant determinant for the prevalence of hepatocellular carcinoma in HCV patients.
  • Thumbnail Image
    Item
    Association of Apelin Genetic Variants with Type Two Diabetes Mellitus in Egyptian Population.
    (A SciTechnol Journal, 2019) Abdelhamid, Amr M; Hafez, Mohamed M; Ibrahim, Sherine M
    Background and Aim: Apelin, the newly identified adipokine and the endogenous ligand for the APJ receptor is related to obesity and insulin resistance. The aim of the study was to investigate the association of 2 single-nucleotide polymorphisms (SNPs) in the apelin gene (APLN) with susceptibility to Type Two Diabetes Mellitus (T2DM) in the Egyptian population. Methods: Two SNPs on APLN were genotyped in 145 diabetic patients and 135 nondiabetic individuals, aged 40-60 years. Realtime polymerase chain reaction (RT-PCR) was used to analyze the 2 SNPs in both the diabetic and the healthy subjects. The association of the 2 SNPs (rs2281068 and rs3115759) in APLN and T2DM risk was investigated. Allele and genotype frequencies between patients and control groups were compared using the Chisquare (χ2 ) test. Results: In the apelin gene; GT/TT genotype of apelin risk genotypes of the rs2281068 variants was found to be significantly related with the risk of T2DM with the power (OR : 9.623, CI : 35.52 - 16.77) (P ≤ 0.001). while on the contrary, the GA/AA genotype of the rs3115759 variants was not at increased risk for T2DM (OR :1.25, CI : 0.785-2.09) (P=0.3408). Conclusions: Both association and functional studies suggested that SNP rs2281068 in APLN is associated with the risk of T2DM in the Egyptian population.