Browsing by Author "Habib I.H.I."

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Lanthanide-DNA probe for spectrofluorimetric determination of some 6-fluoroquinolones in eye-ear pharmaceutical preparations
(Elsevier Inc., 2019) Rizk M.; Habib I.H.I.; Mohamed D.; Mowaka S.; El-Eryan R.T.; Department of Analytical Chemistry; Faculty of Pharmacy; Helwan University; Ein-Helwan; 11745; Egypt; Department of Applied Organic Chemistry; Microanalytical Chemistry Laboratory; National Research Centre; Dokki; Giza; 12622; Egypt; Department of Pharmaceutical Analytical Chemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts; October City 6; 11787; Egypt; Department of Analytical Chemistry; Faculty of Pharmacy; British University in Egypt; El-Sherouk City; 11837; Egypt
A sensitive, accurate and precise spectrofluorimetric method was established for the quantification of ciprofloxacin HCl and norfloxacin drugs. The spectrofluorimetric method has involved the development of a ternary complex between the investigated drugs and Tb3+ in presence of Calf thymus DNA which was used as a fluorescence enhancing agent causing reduction in the non-radiative energy loss produced by O[sbnd]H vibration of H2O in the original Tb3+�drug complex. Thus, the fluorescence of the system (Tb3+�drug�DNA) at ?em of 545 nm for ?ex of 272 nm was enhanced. Investigation of all the conditions that could affect the reaction as well as the formed complexes' spectral properties was performed. Upon utilization of the optimum reaction conditions; the spectrofluorimetric method was highly sensitive and showed linearity over the concentration range of 40�700 ng/mL for both ciprofloxacin and norfloxacin with a correlation coefficient of 0.9998 and 0.9997, respectively. The limit of detection was 12.56 ng/mL and 11.44 ng/mL, while the limit of quantification was 38.06 ng/mL and 34.66 ng/mL for ciprofloxacin and norfloxacin, respectively. The developed method was effectively utilized for the estimation of the studied drugs in eye-ear pharmaceutical dosage forms. The achieved results were compared statistically with those of the pharmacopoeial method where no significant difference was perceived concerning both accuracy and precision. 2019 Elsevier B.V.
Square wave voltammetric determination of rasagiline mesylate on hanging mercury drop electrode and its application in dosage form and biological fluids
(Institute of Electrical and Electronics Engineers Inc., 2016) Habib I.H.I.; Rizk M.; Mohamed D.; Mowaka S.; El-Eryan R.T.; Microanalytical Chemistry Laboratory; Applied Organic Chemistry Department; National Research Centre; Dokki; Giza; 12622; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Helwan University; Cairo; 11745; Egypt; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts; 6 October City; 11787; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; British University in Egypt; El-Sherouk City; 11837; Egypt
A new square wave voltammetric (SWV) method has been developed and validated for the determination of Rasagiline mesylate in bulk, dosage form and human plasma due to a cathodic behaviour of its acetylene group at Hanging Mercury Drop Electrode (HMDE). Many facts such as different supporting electrolytes, scan rate, pulse amplitude, accumulation time and accumulation potential were studied. A good linearity was obtained over a concentration range of (2.8 x10-8 to1.4 x10-7mol L-1) with 99.78% as mean recovery and 1.09%, as the relative standard deviation and limits of detection and quantification were found to be (2.7 x10-9 and 9.24 x1 0-9mol L-1), respectively. The accuracy and precision of the method were within acceptable limits. The method was applied for determining the active ingredients in its tablets with % recoveries � relative standard deviation of 95.46 � 3.7 6 and in spiked human plasma with 94.3 � 2.8 9%, respectively. � 2016 The Authors.